Work with LifeSpan to design a custom immunohistochemistry to address your specific biological question. Outsource the entire localization process without having to
worry about finding and characterizing target specific antibodies, sourcing and validating difficult-to-find tissues, and having the ability to interpret the resulting
immunostaining in relation to complex human pathologies.
TCR Screening Services
Test your therapeutic antibodies in immunohistochemistry against a broad panel of normal frozen human tissue types in order to determine potential unintended binding.
Our non-GLP TCR services are designed on the FDA recommendation outlined in their "Points to Consider in the Manufacture and Testing of Monoclonal Antibody Products for Human Use".
BCL2L2 / Bcl-w is a member of the BCL-2 protein family. The proteins of this family form hetero- or homodimers and act as anti- and pro-apoptotic regulators. Expression of this gene in cells has been shown to contribute to reduced cell apoptosis under cytotoxic conditions. Studies of the related gene in mice indicated a role in the survival of NGF- and BDNF-dependent neurons. Mutation and knockout studies of the mouse gene demonstrated an essential role in adult spermatogenesis.
Formalin-fixed and paraffin-embedded human breast carcinoma tissue reacted with Bcl-w BH3 Domain Antibody , which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.
Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. BC = breast carcinoma; HC = hepatocarcinoma.
BCLW Antibody (BH3) (RB00972) western blot of mouse NIH-3T3 cell line lysates (35 ug/lane). The BCLW antibody detected the BCLW protein (arrow).
Western blot of Bcl-w3 (arrow) using rabbit polyclonal Bcl-w BH3 Domain Antibody. 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected with the BCL2L2 gene (Lane 2) (Origene Technologies).
Western blot of anti-Bcl-w BH3 domain antibody in HL-60 cell lysate. Bcl-w BH3 domain (arrow) was detected using purified antibody. Secondary HRP-anti-rabbit was used for signal visualization with chemiluminescence.
Requested From: United States
Date Requested: 12/8/2016