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Work with LifeSpan to design a custom immunohistochemistry to address your specific biological question. Outsource the entire localization process without having to worry about finding and characterizing target specific antibodies, sourcing and validating difficult-to-find tissues, and having the ability to interpret the resulting immunostaining in relation to complex human pathologies.

Test your therapeutic antibodies in immunohistochemistry against a broad panel of normal frozen human tissue types in order to determine potential unintended binding. Our non-GLP TCR services are designed on the FDA recommendation outlined in their "Points to Consider in the Manufacture and Testing of Monoclonal Antibody Products for Human Use".

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BCL2L10 / Diva Antibody (N‑Terminus) IHC‑plus™ LS‑B452 Best Seller
Note: This antibody replaces LS-C19477
Diva antibody LS-B452 is an unconjugated rabbit polyclonal antibody to human Diva (BCL2L10). Validated for ICC, IHC and WB. Tested on 20 paraffin-embedded human tissues. Cited in 1 publication.
Catalog
Size
Price
LS-B452-50
50 µg (1 mg/ml)
Unavailable

Popular BCL2L10 / Diva Products

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Product Description

Diva antibody LS-B452 is an unconjugated rabbit polyclonal antibody to human Diva (BCL2L10). Validated for ICC, IHC and WB. Tested on 20 paraffin-embedded human tissues. Cited in 1 publication.
About BCL2L10 / Diva
BCL2L10 / Diva belongs to the BCL-2 protein family. BCL-2 family members form hetero- or homodimers and act as anti- or pro-apoptotic regulators that are involved in a wide variety of cellular activities. The protein encoded by this gene contains conserved BH4, BH1 and BH2 domains. This protein can interact with other members of BCL-2 protein family including BCL2, BCL2L1/BCL-X(L), and BAX. Q9HD36 NM_020396 NP_065129.1

BCL2L10 Antibody, BCL-B Antibody, Apoptosis regulator Bcl-B Antibody, BCLB Antibody, Bcl2-L-10 Antibody, Diva not vaccine Antibody, Anti-apoptotic protein NrH Antibody, Bcl-2-like protein 10 Antibody, Boo Antibody, Boo not bladder Antibody, Diva Antibody


Specifications

Target
Human BCL2L10 / Diva
Host
Rabbit
Reactivity
Human (tested or 100% immunogen sequence identity)
Clonality
IgG Polyclonal
Conjugations
Unconjugated
Purification
Immunoaffinity purified
Modifications
Unmodified
Applications
  • IHC
  • IHC - Paraffin (5 µg/ml)
  • ICC
  • Western blot (1 - 2 µg/ml)
Performing IHC? See our complete line of Immunohistochemistry Reagents including antigen retrieval solutions, blocking agents ABC Detection Kits and polymers, biotinylated secondary antibodies, substrates and more.
Blocking Peptide
LS-E30027 - Liquid - 50 µg - $145.00
Immunizing peptide used to generate LS-B452. Useful for pre-absorption and neutralization of the antibody's antigen binding site.
Epitope
N-Terminus
Usage
Immunohistochemistry: LS-B452 was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for LS-B452 was determined to be 5 ug/ml.
Presentation
PBS, 0.02% sodium azide.
Storage
Store at 4°C for 3 months and -20°C, stable for up to 1 year. Avoid repeated freeze-thaw cycles.
Restrictions
For research use only.
Guarantee
This antibody carries the LSBio 100% Guarantee.

Publications (1)

1
Loss of BCL2L10 protein expression as prognostic predictor for poor clinical outcome in gastric carcinoma. Xu JD, Furuya T, Cao XX, Liu XL, Li QQ, Wang WJ, Xu JW, Xu ZD, Sasaki K, Liu XP. Histopathology. 2010 Dec;57:814-24. [Full Text Article] [PubMed:21166696]

Customer Reviews (0)


Images


Immunohistochemistry

Anti-BCL2L10 / BCL-B antibody IHC of human brain, cortex. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B452 concentration 5 ug/ml.
Anti-BCL2L10 / BCL-B antibody IHC of human brain, cortex. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B452 concentration 5 ug/ml.

Immunocytochemistry

Immunocytochemistry of Bcl-B in Jurkat cells with Bcl-B antibody at 10 µg/mL.
Immunocytochemistry of Bcl-B in Jurkat cells with Bcl-B antibody at 10 µg/mL.

Immunocytochemistry

Immunocytochemistry of Bcl-B in Jurkat cells with Bcl-B antibody at 10 ug/ml.
Immunocytochemistry of Bcl-B in Jurkat cells with Bcl-B antibody at 10 ug/ml.

Western blot

Western blot analysis of Bcl-B in Jurkat cell lysate with Bcl-B antibody at (A) 1 and (B) 2 µg/mL.
Western blot analysis of Bcl-B in Jurkat cell lysate with Bcl-B antibody at (A) 1 and (B) 2 µg/mL.

Western blot

Western blot of Bcl-B in Jurkat lysate with Bcl-B antibody at 1 ug/ml in the (A) absence and (B) presence of blocking peptide.
Western blot of Bcl-B in Jurkat lysate with Bcl-B antibody at 1 ug/ml in the (A) absence and (B) presence of blocking peptide.

Immunohistochemistry

Anti-BCL2L10 / BCL-B antibody IHC of human brain, cortex. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B452 concentration 5 ug/ml.
Anti-BCL2L10 / BCL-B antibody IHC of human brain, cortex. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B452 concentration 5 ug/ml.

Immunocytochemistry

Immunocytochemistry of Bcl-B in Jurkat cells with Bcl-B antibody at 10 µg/mL.
Immunocytochemistry of Bcl-B in Jurkat cells with Bcl-B antibody at 10 µg/mL.

Immunocytochemistry

Immunocytochemistry of Bcl-B in Jurkat cells with Bcl-B antibody at 10 ug/ml.
Immunocytochemistry of Bcl-B in Jurkat cells with Bcl-B antibody at 10 ug/ml.

Western blot

Western blot analysis of Bcl-B in Jurkat cell lysate with Bcl-B antibody at (A) 1 and (B) 2 µg/mL.
Western blot analysis of Bcl-B in Jurkat cell lysate with Bcl-B antibody at (A) 1 and (B) 2 µg/mL.

Western blot

Western blot of Bcl-B in Jurkat lysate with Bcl-B antibody at 1 ug/ml in the (A) absence and (B) presence of blocking peptide.
Western blot of Bcl-B in Jurkat lysate with Bcl-B antibody at 1 ug/ml in the (A) absence and (B) presence of blocking peptide.

Immunohistochemistry

Anti-BCL2L10 / BCL-B antibody IHC of human brain, cortex. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B452 concentration 5 ug/ml.
Anti-BCL2L10 / BCL-B antibody IHC of human brain, cortex. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B452 concentration 5 ug/ml.

Immunocytochemistry

Immunocytochemistry of Bcl-B in Jurkat cells with Bcl-B antibody at 10 µg/mL.
Immunocytochemistry of Bcl-B in Jurkat cells with Bcl-B antibody at 10 µg/mL.

Immunocytochemistry

Immunocytochemistry of Bcl-B in Jurkat cells with Bcl-B antibody at 10 ug/ml.
Immunocytochemistry of Bcl-B in Jurkat cells with Bcl-B antibody at 10 ug/ml.

Western blot

Western blot analysis of Bcl-B in Jurkat cell lysate with Bcl-B antibody at (A) 1 and (B) 2 µg/mL.
Western blot analysis of Bcl-B in Jurkat cell lysate with Bcl-B antibody at (A) 1 and (B) 2 µg/mL.

Western blot

Western blot of Bcl-B in Jurkat lysate with Bcl-B antibody at 1 ug/ml in the (A) absence and (B) presence of blocking peptide.
Western blot of Bcl-B in Jurkat lysate with Bcl-B antibody at 1 ug/ml in the (A) absence and (B) presence of blocking peptide.

Immunohistochemistry

Anti-BCL2L10 / BCL-B antibody IHC of human brain, cortex. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B452 concentration 5 ug/ml.
Anti-BCL2L10 / BCL-B antibody IHC of human brain, cortex. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B452 concentration 5 ug/ml.

Immunocytochemistry

Immunocytochemistry of Bcl-B in Jurkat cells with Bcl-B antibody at 10 µg/mL.
Immunocytochemistry of Bcl-B in Jurkat cells with Bcl-B antibody at 10 µg/mL.

Immunocytochemistry

Immunocytochemistry of Bcl-B in Jurkat cells with Bcl-B antibody at 10 ug/ml.
Immunocytochemistry of Bcl-B in Jurkat cells with Bcl-B antibody at 10 ug/ml.

Western blot

Western blot analysis of Bcl-B in Jurkat cell lysate with Bcl-B antibody at (A) 1 and (B) 2 µg/mL.
Western blot analysis of Bcl-B in Jurkat cell lysate with Bcl-B antibody at (A) 1 and (B) 2 µg/mL.

Western blot

Western blot of Bcl-B in Jurkat lysate with Bcl-B antibody at 1 ug/ml in the (A) absence and (B) presence of blocking peptide.
Western blot of Bcl-B in Jurkat lysate with Bcl-B antibody at 1 ug/ml in the (A) absence and (B) presence of blocking peptide.


Requested From: 
Date Requested: 6/22/2018

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