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Anti-BCL11A Antibody (N-Terminus) IHC-plus™ LS-B3530

Note: This antibody replaces LS-C101750

Ordering

Wt. Vol. Conc. Price
50 µg 50 µl 1 mg/ml $450
Inquire for larger quantities

LSBio (Direct) LSBio (Direct)
206-374-1102
866-206-6909
Orders@LSBio.com
 

Most Popular BCL11A Antibodies

Anti-BCL11A Antibody (N-Terminus) IHC-plus™ LS-B3530
Rabbit Polyclonal (IgG) to Human BCL11A
Human, Monkey, Mouse, Rat, Bat, Bovine, Dog, Horse, Pig, Chicken, Zebrafish
IHC - Paraffin, Western blot
Unconjugated
Immunohistochemistry Image
Anti-BCL11A Antibody (clone 3D9) IHC-plus™ LS-B6033
Mouse Monoclonal [clone 3D9] (IgG2a,k) to Human BCL11A
Human
IHC - Paraffin, Western blot, ELISA
Unconjugated
Immunohistochemistry Image

100% Guaranteed 100% Guaranteed
Rabbit Polyclonal (IgG) to Human BCL11A
Human, Monkey, Mouse, Rat, Bat, Bovine, Dog, Horse, Pig, Chicken, Zebrafish
IHC - Paraffin, Western blot (applications tested for the base form of this product only)
Unconjugated

Details

Human BCL11A
Rabbit
Human, Monkey, Mouse, Rat, Bat, Bovine, Dog, Horse, Pig, Chicken, Zebrafish (tested or 100% immunogen sequence identity)
IgG Polyclonal
Unconjugated. Also available conjugated with Biotin, FITC, HRP.
Immunoaffinity purified
Unmodified

Applications

  • IHC - Paraffin (5 - 10 µg/ml)
  • Western blot (0.2 - 1 µg/ml)
  • (applications tested for the base form of this product only)

Specificity and Use

BCL11A antibody was raised against synthetic peptide from N-Terminus of human BCL11A (Q9H165, NP_075044). Percent identity by BLAST analysis: Human, Chimpanzee, Gorilla, Gibbon, Monkey, Galago, Marmoset, Mouse, Rat, Elephant, Panda, Dog, Bovine, Bat, Horse, Pig, Chicken, Platypus, Zebrafish (100%); Xenopus (85%).
LS-E14195 - Lyophilized - 100 µg - $145.00
N-Terminus
Human BCL11A
Immunohistochemistry: LS-B3530 was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for LS-B3530 was determined to be 5 ug/ml. Western Blot: Suggested dilution at 1 ug/ml in 5% skim milk / PBS buffer, and HRP conjugated anti-Rabbit IgG should be diluted in 1:50000 - 100000 as second antibody. ELISA titer in peptide based assay: 1:1562500.

Packaging

Lyophilized from PBS with 2% sucrose
50 µl Distilled water
Long term: -20°C, the use of 50% glycerol is recommended if storing aliquots in -20°C for long term use (up to 1 year); Short term (less than one week): +4°C. Avoid repeat freeze-thaw cycles.
For research use only.

About BCL11A

Q9H165 NM_022893 NP_075044.2

BCL11A Antibody, B-cell CLL/lymphoma 11A Antibody, BCL11A-L Antibody, BCL11A-XL Antibody, BCL11a-M Antibody, B-cell lymphoma/leukemia 11A Antibody, BCL-11A Antibody, BCL11A-S Antibody, CTIP1 Antibody, COUP-TF-interacting protein 1 Antibody, HBFQTL5 Antibody, EVI-9 Antibody, KIAA1809 Antibody, ZNF856 Antibody, C2H2-type zinc finger protein Antibody, EVI9 Antibody, Zinc finger protein 856 Antibody

BCL11A is a C2H2 type zinc-finger protein by its similarity to the mouse Bcl11a/Evi9 protein. The corresponding mouse gene is a common site of retroviral integration in myeloid leukemia, and may function as a leukemia disease gene, in part, through its interaction with BCL6. During hematopoietic cell differentiation, this gene is down-regulated. It is possibly involved in lymphoma pathogenesis since translocations associated with B-cell malignancies also deregulates its expression.

Immunohistochemistry

Immunohistochemistry
Anti-BCL11A antibody IHC of human thymus. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B3530 concentration 5 ug/ml. This image was taken for the unconjugated form of this product. Other forms have not been tested.

Immunohistochemistry

Immunohistochemistry
Anti-BCL11A antibody IHC of human spleen. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B3530 concentration 5 ug/ml. This image was taken for the unconjugated form of this product. Other forms have not been tested.

Immunohistochemistry

Immunohistochemistry
Anti-BCL11A antibody IHC of human prostate. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B3530 concentration 5 ug/ml. This image was taken for the unconjugated form of this product. Other forms have not been tested.

Western blot

Western blot
BCL11A antibody LS-B3530 Western blot of Fetal Heart lysate. This image was taken for the unconjugated form of this product. Other forms have not been tested.

Requested From: United States
Date Requested: 12/3/2016

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