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Work with LifeSpan to design a custom immunohistochemistry to address your specific biological question. Outsource the entire localization process without having to worry about finding and characterizing target specific antibodies, sourcing and validating difficult-to-find tissues, and having the ability to interpret the resulting immunostaining in relation to complex human pathologies.

Test your therapeutic antibodies in immunohistochemistry against a broad panel of normal frozen human tissue types in order to determine potential unintended binding. Our non-GLP TCR services are designed on the FDA recommendation outlined in their "Points to Consider in the Manufacture and Testing of Monoclonal Antibody Products for Human Use".

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BBC3 / PUMA Antibody (aa150‑193) LS‑C2857
PUMA antibody LS-C2857 is an unconjugated rabbit polyclonal antibody to human PUMA (BBC3). Validated for WB.
Catalog
Size
Price
LS-C2857-100
100 µl
$335

Popular BBC3 / PUMA Products

Anti-BBC3 / PUMA antibody IHC of human breast. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B1334 concentration 10 ug/ml.
Species: Human, Mouse
Applications: IHC, IHC - Paraffin, ICC, Immunofluorescence, Western blot
Formalin-fixed and paraffin-embedded human breast carcinoma tissue reacted with Puma BH3 Domain antibody , which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.
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PUMA expression in human dysplastic nevi (premalignant melanoma).
Species: Human
Applications: IHC, IHC - Paraffin, Western blot
Antibody
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Applications: Western blot
Immunohistochemistry of paraffin-embedded human mammary cancer using BBC3 antibodyat dilution of 1:100 (40x lens).
Species: Human, Mouse, Rat
Applications: IHC, Immunofluorescence, Western blot, Flow Cytometry

Product Description

BBC3 / PUMA Antibody (aa150-193) for WB/Western LS-C2857

Specifications

Target
Human BBC3 / PUMA
Synonyms
BBC3, Bcl-2-binding component 3, BCL2 binding component 3, JFY-1, JFY1, PUMA, PUMA alpha
Host
Rabbit
Reactivity
Human (tested or 100% immunogen sequence identity)
Clonality
Polyclonal
Conjugations
Unconjugated. Also available conjugated with Biotin, DY650, DY488, DY550.
Purification
Immunoaffinity purified
Modifications
Unmodified
Applications
  • Western blot (2 - 4 µg/ml)
  • (applications tested for the base form of this product only)
Immunogen
BBC3 / PUMA antibody was raised against a synthetic peptide made to a region within the C-terminus of the human PUMA protein sequence (between residues 150-193)
Epitope
aa150-193
Specificity
PUMA.
Presentation
Serum
Storage
Short term: 4°C. Long term: Store at -20°C. Avoid freeze-thaw cycles.
Restrictions
For research use only.
About BBC3 / PUMA
Apoptosis is related to many diseases and development. The p53 tumor-suppressor protein induces apoptosis through transcriptional activation of several genes. A novel p53 inducible pro-apoptotic gene was identified recently and designated PUMA (for p53 upregulated modulator of apoptosis) and bbc3 (for Bcl-2 binding component 3) in human and mouse. PUMA/bbc3 is one of the pro-apoptotic Bcl-2 family members including Bax and Noxa, which are also transcriptional targets of p53. Q9BXH1 NM_014417 NP_055232.1


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Images

Western blot

Detection of the beta isoform of PUMA in HL-60 whole cell lysate using LS-C2857(4 ug/ml). ECL detection at a 30 minute exposure.  This image was taken for the unconjugated form of this product. Other forms have not been tested.
Detection of the beta isoform of PUMA in HL-60 whole cell lysate using LS-C2857(4 ug/ml). ECL detection at a 30 minute exposure. This image was taken for the unconjugated form of this product. Other forms have not been tested.

Western blot

Detection of the beta isoform of PUMA in HL-60 whole cell lysate using LS-C2857(4 ug/ml). ECL detection at a 30 minute exposure.  This image was taken for the unconjugated form of this product. Other forms have not been tested.
Detection of the beta isoform of PUMA in HL-60 whole cell lysate using LS-C2857(4 ug/ml). ECL detection at a 30 minute exposure. This image was taken for the unconjugated form of this product. Other forms have not been tested.

Western blot

Detection of the beta isoform of PUMA in HL-60 whole cell lysate using LS-C2857(4 ug/ml). ECL detection at a 30 minute exposure.  This image was taken for the unconjugated form of this product. Other forms have not been tested.
Detection of the beta isoform of PUMA in HL-60 whole cell lysate using LS-C2857(4 ug/ml). ECL detection at a 30 minute exposure. This image was taken for the unconjugated form of this product. Other forms have not been tested.

Western blot

Detection of the beta isoform of PUMA in HL-60 whole cell lysate using LS-C2857(4 ug/ml). ECL detection at a 30 minute exposure.  This image was taken for the unconjugated form of this product. Other forms have not been tested.
Detection of the beta isoform of PUMA in HL-60 whole cell lysate using LS-C2857(4 ug/ml). ECL detection at a 30 minute exposure. This image was taken for the unconjugated form of this product. Other forms have not been tested.

Requested From: United States
Date Requested: 10/16/2018

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