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Anti-BAP / SIL1 Antibody (aa448-461) IHC-plus™ LS-B3286

Note: This antibody replaces LS-C54570

Ordering

Wt. Vol. Conc. Price
50 µg - 0.5 mg/ml Unavailable

Most Popular BAP / SIL1 Antibodies

Anti-BAP / SIL1 Antibody (aa448-461) IHC-plus™ LS-B3286
Goat Polyclonal to Human BAP / SIL1
Human
IHC - Paraffin, Western blot, ELISA
Unconjugated
Immunohistochemistry Image
Anti-BAP / SIL1 Antibody (clone 3E3) LS-C115047
Mouse Monoclonal [clone 3E3] (IgG1) to Human BAP / SIL1
Human
IHC - Paraffin, Immunofluorescence, Western blot, Flow Cytometry
Unconjugated
Immunohistochemistry Image
Anti-BAP / SIL1 Antibody (clone 1C4) LS-C115086
Mouse Monoclonal [clone 1C4] (IgG1) to Human BAP / SIL1
Human
IHC - Paraffin, Immunofluorescence, Western blot
Unconjugated
Immunohistochemistry Image

100% Guaranteed 100% Guaranteed
Goat Polyclonal to Human BAP / SIL1
Human
IHC - Paraffin, Western blot, ELISA
Unconjugated

Details

Human BAP / SIL1
Goat
Human (tested or 100% immunogen sequence identity)
Monkey, Bat, Dog, Horse (at least 90% immunogen sequence identity)
Polyclonal
Unconjugated
Immunoaffinity purified
Unmodified

Applications

  • IHC - Paraffin (5 µg/ml)
  • Western blot (1 - 2 µg/ml)
  • ELISA (1:8000)

Specificity and Use

BAP / SIL1 antibody was raised against synthetic peptide C-ELLGSVNSLLKELR from the C-terminus of human SIL1 (NP_071909.1; NP_001032722.1). Percent identity by BLAST analysis: Human, Gorilla, Monkey (100%); Marmoset, Dog, Bat, Horse (93%); Hamster, Panda, Rabbit (86%).
LS-E25591 - Lyophilized - 100 µg - $145.00
Immunizing peptide used to generate LS-B3286. Useful for pre-absorption and neutralization of the antibody's antigen binding site.
aa448-461
Human SIL1. Variants (NP_071909.1; NP_001032722.1) encode the same isoform.
Immunohistochemistry: LS-B3286 was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for LS-B3286 was determined to be 5 ug/ml.

Packaging

Tris-buffered saline, pH 7.3, 0.5% BSA, 0.02% sodium azide
Store at -20°C. Minimize freezing and thawing.
For research use only.

About BAP / SIL1

Q9H173 NM_022464 NP_071909.1

SIL1 Antibody, BiP-associated protein Antibody, BAP Antibody, Marinesco-Sjogren syndrome Antibody, MSS Antibody, ULG5 Antibody

BAP / SIL1 is a resident endoplasmic reticulum (ER), N-linked glycoprotein with an N-terminal ER targeting sequence, 2 putative N-glycosylation sites, and a C-terminal ER retention signal. This protein functions as a nucleotide exchange factor for another unfolded protein response protein. Mutations in this gene have been associated with Marinesco-Sjogren syndrome. Alternate transcriptional splice variants have been characterized.

Immunohistochemistry

Immunohistochemistry
Anti-SIL1 antibody IHC of human testis. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B3286 concentration 5 ug/ml.

Immunohistochemistry

Immunohistochemistry
Anti-SIL1 antibody IHC of human liver. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B3286 concentration 5 ug/ml.

Western blot

Western blot
Antibody staining (1 ug/ml) of Human Kidney lysate (RIPA buffer, 30 ug total protein per lane). Primary incubated for 1 hour. Detected by Western blot of chemiluminescence.
 

Requested From: 
Date Requested: 3/28/2017

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