B cell

Mouse

Rat (tested or 100% immunogen sequence identity)

Monoclonal

Ki-B1R

Unconjugated

Affinity purified

Unmodified

Lymphocytes

Ki-B1R recognizes a surface antigen of 47kDa molecular weight, which appears as a single band under reducing conditions in SDS-PAGE. The epitope has not been further characterized. Antigen distribution: Isolated cells: In a test comparing the immunophenotype of rat blood lymphocytes, Ki-B1R recognised 19% + 3% of peripheral blood lymphocytes (n=5). In the same test, 18% +/- 3% of the lymphocytes stained positive for sIg, whereas 79% +/- 3% stained positive with Ki-T1R, the rat pan T-cell marker. Tissue sections: Ki-B1R shows a staining pattern similar to that of anti-rat immunoglobulin markers. Follicular mantle B- lymphocytes are strongly positive whereas staining of germinal centre B-lymphocytes depends on the immunological status of the lyphoid organ. In the gastrointestinal tract, the typical mucosa-associated lymphoid tissue (MALT) selectively displays the B-follicles, mostly those containing prominent germinal centres. The reactivity pattern of Ki-B1R does not change when applied in vivo. Specificity: Rat: mature B-lymphocytes, plasma cells. Other species: not tested.

• IHC - Paraffin (1:10)
• IHC - Frozen (1:400)

Paraffin sections: 1:10; no antigen retrieval required, though microwave treatment may enhance the reactivity.

Lyophilized from PBS, pH 7.2, 5 mg/ml BSA, 0.09% Sodium Azide

Reconstitute with 0.5ml distilled H2O.

Store lyophilized at 4°C. Once reconstituted, aliquot and store at -20°C. Avoid freeze/thaw cycles. Store undiluted.

For research use only. Intended for use by laboratory professionals.

This antibody carries the LSBio 100% Guarantee.