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Anti-ATG8 / Autophagy 8 Antibody LS-C59543


Wt. Vol. Conc. Price
500 µg - 5 mg/ml Unavailable

Most Popular ATG8 / Autophagy 8 Antibodies

Anti-ATG8 / Autophagy 8 Antibody LS-C59543
Rabbit Polyclonal to Yeast ATG8 / Autophagy 8
Western blot, ELISA
Western blot Image
Anti-ATG8 / Autophagy 8 Antibody (FITC) LS-C394282
Rabbit Polyclonal (IgG) to Candida glabrata ATG8 / Autophagy 8
Candida glabrata
FITC Conjugated
Anti-ATG8 / Autophagy 8 Antibody (Biotin) LS-C394286
Rabbit Polyclonal (IgG) to Candida glabrata ATG8 / Autophagy 8
Candida glabrata
Biotin Conjugated

100% Guaranteed 100% Guaranteed
Rabbit Polyclonal to Yeast ATG8 / Autophagy 8
Western blot, ELISA


Yeast ATG8 / Autophagy 8
Yeast (tested or 100% immunogen sequence identity)
Ion exchange chromatography


  • Western blot (1:4000 - 1:8000)
  • ELISA (1:20000 - 1:100000)

Specificity and Use

Recombinant yeast APG8 protein.
anti- Rabbit Serum
This purified polyclonal antibody reacts with yeast APG8 by western blot and ELISA. Although not tested, this antibody is likely functional in immunohistochemistry and immunoprecipitation. This antibody using the specified conditions may recognize other prominent intrinsic bands (UBLs or their conjugates). Other intrinsic bands are readily detectable in yeast lysates at lower antibody dilutions. A 14 kD band corresponding to yeast APG8 is detected. Most yeast cell lysates can be used as a positive control without induction or stimulation.


0.02 M potassium phosphate, 0.15 M sodium chloride, pH 7.2, 0.01% sodium azide.
deionized water
Store lyophilized at 4°C. Once reconstituted, aliquot and store at -20°C. Avoid freeze-thaw cycles.
For research use only.

About ATG8 / Autophagy 8

Western blot

Western blot
Anti-APG8 Antibody - Western Blot. Western blot of APG8 fusion protein. Anti-APG8 antibody generated by immunization with recombinant yeast APG8 was tested by western blot with other anti-UBL antibodies against E. coli lysates expressing the APG8-GFP fusion protein. All UBLs possess limited homology to Ubiquitin and to each other, therefore it is important to know the degree of reactivity of each antibody against each UBL. Panel A shows total protein staining using ponceau. Panel B shows specific reaction with APG8 using a 1:4000 and 1:8000 dilution of IgG fraction of Rabbit-anti-APG8 (Yeast) followed by reaction with a 1:15000 dilution of HRP Goat-a-Rabbit IgG MX (code # LS-C60865). All primary antibodies were diluted in TTBS buffer supplemented with 5% non-fat milk and incubated with the membranes overnight at 4° C. E. coli lysate proteins were separated by SDS-PAGE using a 15% gel. Similar experiments (data not shown), where other UBL fusion proteins were separated and probed with this antibody showed no reactivity of anti-APG8 with other UBLs. This data indicates that anti-APG8 is highly specific and does not cross react with other UBLs. A chemiluminescence system was used for signal detection (Roche). Other detection systems will yield similar results. Data contributed by M. Malakhov, www. lifesensors. com, personal communication.

Requested From: 
Date Requested: 3/24/2017

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