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Anti-ATF3 Antibody (aa113-130) IHC-plus™ LS-B329

Note: This antibody replaces LS-C18781
Catalog Size Price
LS-B329-50 50 µg (1 mg/ml) Unavailable

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100% Guaranteed Publications (1)
Rabbit Polyclonal to Human ATF3
Human
IHC - Paraffin, Western blot, ELISA
Unconjugated

Details

Human ATF3
Rabbit
Human (tested or 100% immunogen sequence identity)
Polyclonal
Unconjugated
Affinity purified
Unmodified

Applications

  • IHC - Paraffin (5 µg/ml)
  • Western blot (1:200)
  • ELISA

Specificity and Use

ATF3 antibody was raised against synthetic peptide from human ATF3.
aa113-130
This antibody was produced from a aa 113-130 of human ATF3.
Immunohistochemistry: LS-B329 was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for LS-B329 was determined to be 5 ug/ml.

Packaging

0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2 with 0.01% (w/v) Sodium Azide
+4°C or -20°C, Avoid repeated freezing and thawing.
For research use only.

Publications (1)

1
Opposing roles for calcineurin and ATF3 in squamous skin cancer. Wu X, Nguyen BC, Dziunycz P, Chang S, Brooks Y, Lefort K, Hofbauer GF, Dotto GP. Nature. 2010 May;465:368-72. [Full Text Article] [PubMed:20485437] [PMC:PMC3050632]

About ATF3

P18847 NM_001674 NP_004015.4

ATF3 Antibody

Activating transcription factor 3 (ATF3)is a member of the mammalian activation transcription factor/cAMP responsive element-binding (CREB) protein family of transcription factors. It encodes a protein with a calculated molecular mass of 22 kD. ATF3 represses rather than activates transcription from promoters with ATF binding elements.

Immunohistochemistry

Immunohistochemistry
Anti-ATF-3 antibody IHC of human prostate. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B329 concentration 5 ug/ml.

Western blot

Western blot
Anti-ATF3 Antibody - Western Blot. Western blot of E. coli whole cell extract transfected with GST epitope tagged human ATF3. Affinity purified anti-ATF3 detects a band ~48 kD corresponding to recombinant human ATF3. Immunostaining using anti-GST epitope tag antibody confirms the composition of the recombinant band (not shown). The protein was transferred to nitrocellulose using standard methods. After blocking with 5% goat serum and 0.5% non fat milk in PBS, the membrane was probed with the primary antibody diluted 1:200 in 0.2X blocking buffer in PBS overnight at 4°C. Reaction was followed by washes and reaction with a 1:5000 dilution of IRDye800 conjugated Gt-a-Rabbit IgG [H&L] (code for 30 min at room temperature. LICORs Odyssey Infrared Imaging System was used to scan and process the image. Other detection systems will yield similar results.

Western blot

Western blot
Anti-ATF3 Antibody - Western Blot. Western blot of mammalian whole cell extract transfected with HA epitope tagged human ATF3. Affinity purified anti-ATF3 detects a band ~31 kD corresponding to recombinant human ATF3. Immunostaining using anti-HA epitope tag antibody confirms the composition of the recombinant band (not shown). The protein was transferred to nitrocellulose in 30 minutes using standard methods. After blocking with 5% goat serum and 0.5% non-fat milk in PBS, the membrane was probed with the primary antibody diluted 1:200 in 0.2X blocking buffer in PBS overnight at 4C. Reaction was followed by washes and reaction with a 1:5000 dilution of IRDye800 conjugated Gt-a-Rabbit IgG [H&L] (code for 30 min at room temperature. LICORs Odyssey Infrared Imaging System was used to scan and process the image. Other detection systems will yield similar results.

Requested From: 
Date Requested: 8/17/2017

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