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Anti-ATAD5 / ELG1 Antibody (aa63-76) LS-C19118


Wt. Vol. Conc. Price
100 µg - 0.5 mg/ml Unavailable

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Rabbit Polyclonal to Human ATAD5 / ELG1
Western blot, ELISA


Human ATAD5 / ELG1
Human (tested or 100% immunogen sequence identity)
Immunoaffinity purified


  • Western blot (1:500 - 1:2000)
  • ELISA (1:3000 - 1:15000)

Specificity and Use

ATAD5 / ELG1 antibody was raised against synthetic peptide from human ATAD5.
Amino acids 63-76 of Human Elg 1.
This affinity purified antibody has been tested for use in ELISA and western blot. Specific conditions for reactivity should be optimized by the end user. Expect a band approximately 120 kD in size corresponding to Elg1 by western blotting in the appropriate cell lysate or extract.


0.02 M potassium phosphate, 0.15 M sodium chloride, pH 7.2, 0.01% sodium azide.
Store vial at -20 C prior to opening. Dilute only prior to immediate use. For extended storage aliquot contents and freeze at -20 C or below. Avoid cycles of freezing and thawing.
For research use only.

About ATAD5 / ELG1

Q96QE3 NM_024857 NP_079133.3

ATAD5 Antibody, C17orf41 Antibody, FRAG1 Antibody, ELG1 Antibody

Involved in DNA damage response. Involved in a RAD9A-related damage checkpoint, a pathway that is important in determining whether DNA damage is compatible with cell survival or whether it requires cell elimination by apoptosis. Modulates the RAD9A interaction with BCL2 and thereby induces DNA damages-induced apoptosis.

Western blot

Western blot
Anti-Elg1 Antibody - Western Blot. Western blot of Affinity Purified anti-Elg1 antibody shows detection of a band ~120 kD corresponding to human Elg1 (arrowhead) in various cell lysates. Lanes contain ~5 ug of HeLa nuclear extract (1), HeLa (2), A431 (3), Jurkat (4) and HEK293 (5) whole cell lysates. After SDS-PAGE, transfer and blocking, the membrane was probed with the primary antibody diluted to 1:500. The membrane was then washed and reacted with a HRP conjugated Gt-a-Rabbit IgG [H&L] MX followed by ECL detection using a 2 m exposure time. The expected molecular weight of Elg1 is 120kD according to Kanellis P et al. 2003, although the predicted molecular weight is 207 kD. The 50kD bands in Jurkat and 293 cell lysates are probably cross-reaction with other proteins. Both the 120 kD and 50 kD bands are not observed when antibody is pre-incubated with peptide (data not shown).

Requested From: 
Date Requested: 4/23/2017

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