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Work with LifeSpan to design a custom immunohistochemistry to address your specific biological question. Outsource the entire localization process without having to worry about finding and characterizing target specific antibodies, sourcing and validating difficult-to-find tissues, and having the ability to interpret the resulting immunostaining in relation to complex human pathologies.

Test your therapeutic antibodies in immunohistochemistry against a broad panel of normal frozen human tissue types in order to determine potential unintended binding. Our non-GLP TCR services are designed on the FDA recommendation outlined in their "Points to Consider in the Manufacture and Testing of Monoclonal Antibody Products for Human Use".

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ARPC1B / p41‑ARC / ARP2 Antibody (aa2‑14) IHC‑plus™ LS‑B2744 Best Seller
Note: This antibody replaces LS-C54542, LS-C63592
ARP2 antibody LS-B2744 is an unconjugated goat polyclonal antibody to ARP2 (ARPC1B / p41-ARC) from human, mouse, rat and other species. Validated for IHC, Peptide-ELISA and WB. Tested on 20 paraffin-embedded human tissues.
Catalog
Size
Price
LS-B2744-50
50 µg (0.5 mg/ml)
Unavailable

Popular ARPC1B / p41-ARC / ARP2 Products

Formalin-fixed and paraffin-embedded human lung carcinoma reacted with ARPC1B Antibody , which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.
Species: Human
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Antibody
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Human Spleen: Formalin-Fixed, Paraffin-Embedded (FFPE)
Species: Human, Mouse, Bovine, Dog, Guinea pig, Pig, Rabbit, Chicken
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Species: Human, Mouse, Dog, Guinea pig, Horse, Pig, Rabbit, Xenopus
Applications: IHC, IHC - Paraffin, Western blot, Immunoprecipitation

Product Description

ARP2 antibody LS-B2744 is an unconjugated goat polyclonal antibody to ARP2 (ARPC1B / p41-ARC) from human, mouse, rat and other species. Validated for IHC, Peptide-ELISA and WB. Tested on 20 paraffin-embedded human tissues.
About ARPC1B / p41-ARC / ARP2
ARPC1B / p41-ARC / ARP2 is one of seven subunits of the human Arp2/3 protein complex. This subunit is a member of the SOP2 family of proteins and is most similar to the protein encoded by gene ARPC1A. The similarity between these two proteins suggests that they both may function as p41 subunit of the human Arp2/3 complex that has been implicated in the control of actin polymerization in cells. O15143 NM_005720 NP_005711.1

ARPC1B Antibody, ARC41 Antibody, Arp2/3 complex 41 kd subunit Antibody, Arp2/3 complex 41 kDa subunit Antibody, p41-ARC Antibody, p40-ARC Antibody


Specifications

Target
Human ARPC1B / p41-ARC / ARP2
Host
Goat
Reactivity
Human, Monkey, Mouse, Rat, Bovine, Dog, Hamster, Horse, Pig, Rabbit (tested or 100% immunogen sequence identity)
Predicted
Bat, Chicken, Zebrafish (at least 90% immunogen sequence identity)
Clonality
Polyclonal
Conjugations
Unconjugated
Purification
Immunoaffinity purified
Modifications
Unmodified
Applications
  • IHC
  • IHC - Paraffin (2.5 µg/ml)
  • Western blot (2 - 4 µg/ml)
  • Peptide Enzyme-Linked Immunosorbent Assay (1:32000)
Performing IHC? See our complete line of Immunohistochemistry Reagents including antigen retrieval solutions, blocking agents ABC Detection Kits and polymers, biotinylated secondary antibodies, substrates and more.
Immunogen
Synthetic peptide AYHSFLVEPISCH from the N-terminus of human ARPC1B / p41-ARC (NP_005711.1). Percent identity by BLAST analysis: Human, Gorilla, Gibbon, Marmoset, Mouse, Rat, Hamster, Elephant, Panda, Dog, Bovine, Horse, Rabbit, Pig (100%); Bat, Opossum, Turkey, Chicken, Platypus, Catfish, Salmon, Sablefish, Stickleback, Pufferfish, Zebrafish (92%); Xenopus (85%).
Blocking Peptide
LS-E25358 - Lyophilized - 100 µg - $145.00
Immunizing peptide used to generate LS-B2744. Useful for pre-absorption and neutralization of the antibody's antigen binding site.
Epitope
aa2-14
Specificity
Human ARPC1B / p41-ARC.
Usage
Immunohistochemistry: LS-B2744 was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for LS-B2744 was determined to be 2.5 ug/ml.
Presentation
Tris-buffered saline, pH 7.3, 0.5% BSA, 0.02% sodium azide
Storage
Store at -20°C. Avoid freeze-thaw cycles.
Restrictions
For research use only.
Guarantee
This antibody carries the LSBio 100% Guarantee.

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Images


Immunohistochemistry

Anti-ARPC1B / p41-ARC antibody IHC of human thymus. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B2744 concentration 5 ug/ml.
Anti-ARPC1B / p41-ARC antibody IHC of human thymus. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B2744 concentration 5 ug/ml.

Western blot

Staining (2?g/ml) of Human Liver lysate (RIPA buffer, 35?g total protein per lane). Primary incubated for 1 hour. Detected by chemiluminescence.
Staining (2?g/ml) of Human Liver lysate (RIPA buffer, 35?g total protein per lane). Primary incubated for 1 hour. Detected by chemiluminescence.

Western blot

Antibody staining (2 ug/ml) of Human Liver lysate (RIPA buffer, 35 ug total protein per lane). Primary incubated for 1 hour. Detected by Western blot of chemiluminescence.
Antibody staining (2 ug/ml) of Human Liver lysate (RIPA buffer, 35 ug total protein per lane). Primary incubated for 1 hour. Detected by Western blot of chemiluminescence.

Immunohistochemistry

Anti-ARPC1B / p41-ARC antibody IHC of human thymus. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B2744 concentration 5 ug/ml.
Anti-ARPC1B / p41-ARC antibody IHC of human thymus. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B2744 concentration 5 ug/ml.

Western blot

Staining (2?g/ml) of Human Liver lysate (RIPA buffer, 35?g total protein per lane). Primary incubated for 1 hour. Detected by chemiluminescence.
Staining (2?g/ml) of Human Liver lysate (RIPA buffer, 35?g total protein per lane). Primary incubated for 1 hour. Detected by chemiluminescence.

Western blot

Antibody staining (2 ug/ml) of Human Liver lysate (RIPA buffer, 35 ug total protein per lane). Primary incubated for 1 hour. Detected by Western blot of chemiluminescence.
Antibody staining (2 ug/ml) of Human Liver lysate (RIPA buffer, 35 ug total protein per lane). Primary incubated for 1 hour. Detected by Western blot of chemiluminescence.

Immunohistochemistry

Anti-ARPC1B / p41-ARC antibody IHC of human thymus. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B2744 concentration 5 ug/ml.
Anti-ARPC1B / p41-ARC antibody IHC of human thymus. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B2744 concentration 5 ug/ml.

Western blot

Staining (2?g/ml) of Human Liver lysate (RIPA buffer, 35?g total protein per lane). Primary incubated for 1 hour. Detected by chemiluminescence.
Staining (2?g/ml) of Human Liver lysate (RIPA buffer, 35?g total protein per lane). Primary incubated for 1 hour. Detected by chemiluminescence.

Western blot

Antibody staining (2 ug/ml) of Human Liver lysate (RIPA buffer, 35 ug total protein per lane). Primary incubated for 1 hour. Detected by Western blot of chemiluminescence.
Antibody staining (2 ug/ml) of Human Liver lysate (RIPA buffer, 35 ug total protein per lane). Primary incubated for 1 hour. Detected by Western blot of chemiluminescence.

Immunohistochemistry

Anti-ARPC1B / p41-ARC antibody IHC of human thymus. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B2744 concentration 5 ug/ml.
Anti-ARPC1B / p41-ARC antibody IHC of human thymus. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B2744 concentration 5 ug/ml.

Western blot

Staining (2?g/ml) of Human Liver lysate (RIPA buffer, 35?g total protein per lane). Primary incubated for 1 hour. Detected by chemiluminescence.
Staining (2?g/ml) of Human Liver lysate (RIPA buffer, 35?g total protein per lane). Primary incubated for 1 hour. Detected by chemiluminescence.

Western blot

Antibody staining (2 ug/ml) of Human Liver lysate (RIPA buffer, 35 ug total protein per lane). Primary incubated for 1 hour. Detected by Western blot of chemiluminescence.
Antibody staining (2 ug/ml) of Human Liver lysate (RIPA buffer, 35 ug total protein per lane). Primary incubated for 1 hour. Detected by Western blot of chemiluminescence.


Requested From: 
Date Requested: 6/25/2018

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