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Anti-ARHGDIB / D4 GDI Antibody (clone 10D774) LS-C515

Note: This antibody replaces LS-C64482, LS-C14796

Ordering

Wt. Vol. Conc. Price
100 µg 200 µl 0.5 mg/ml $335
Inquire for larger quantities

LSBio (Direct) LSBio (Direct)
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Mouse Monoclonal [clone 10D774] (IgG) to Human ARHGDIB / D4 GDI
Human, Monkey, Bat, Bovine, Dog, Horse, Pig, Chicken
Western blot, Flow Cytometry
Unconjugated

Details

Human ARHGDIB / D4 GDI
Mouse
Human, Monkey, Bat, Bovine, Dog, Horse, Pig, Chicken (tested or 100% immunogen sequence identity)
Rabbit (at least 90% immunogen sequence identity)
IgG Monoclonal [10D774]
Protein G purified

Applications

Western blot (1 - 2 µg/ml), Flow Cytometry (0.5 - 1 µg/10E6 cells)

Specificity and Use

ARHGDIB / D4 GDI antibody was raised against a synthetic peptide corresponding to amino acids 125-137 (YVQHTYRTGVKVD) of human D4-GDI, GenBank no. ref|NP_001166.3|. The peptide sequence used for immunogen is 100% conserved in human, dog, cow, chick, and horse. Percent identity by BLAST analysis: Human, Chimpanzee, Gorilla, Orangutan, Gibbon, Monkey, Galago, Marmoset, Ferret, Panda, Dog, Cat, Bovine, Horse, Pig, Turkey, Chicken, Lizard (100%); Rabbit, Opossum, Guinea pig (92%); Mouse, Elephant, Zebra finch (85%); Rat (77%).
A 28 kD band is observed.

Packaging

PBS, 0.02% sodium azide, 0.02% gelatin.
Long term: -70°C; Short term: +4°C
For research use only.

About ARHGDIB / D4 GDI

P52566 NM_001175 NP_001166.3

ARHGDIB Antibody, D4 GDI Antibody, D4-GDI Antibody, GDIA2 Antibody, Ly-GDI Antibody, RAP1GN1 Antibody, Rho-GDI beta Antibody, RhoGDI2 Antibody, Rho GDI 2 Antibody, D4 Antibody, GDID4 Antibody, LYGDI Antibody

Members of the Rho (or ARH) protein family (see MIM 165390) and other Ras-related small GTP-binding proteins (see MIM 179520) are involved in diverse cellular events, including cell signaling, proliferation, cytoskeletal organization, and secretion. The GTP-binding proteins are active only in the GTP-bound state.

Western blot

Western blot
Western blot of antibody in whole Jurkat cell lysate.

Flow Cytometry

Flow Cytometry
HL60 cells were left untreated (green) or treated with camptothecin to induce apoptosis (red). Cells were analyzed by intracellular flow cytometry using either the

Requested From: United States
Date Requested: 9/25/2016

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