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Anti-ARA55 / HIC-5 Antibody (aa179-228) IHC-plus™ LS-B1004

Note: This antibody replaces LS-C29954

Ordering

Wt. Vol. Conc. Price
50 µg 50 µl 1 mg/ml $450
Inquire for larger quantities

LSBio (Direct) LSBio (Direct)
206-374-1102
866-206-6909
Orders@LSBio.com
 

Most Popular ARA55 / HIC-5 Antibodies

Anti-ARA55 / HIC-5 Antibody (aa179-228) IHC-plus™ LS-B1004
Rabbit Polyclonal to Human ARA55 / HIC-5
Human, Monkey, Mouse, Rat, Bovine, Goat, Guinea pig, Horse, Rabbit
IHC - Paraffin, Western blot
Unconjugated
Immunohistochemistry Image
Anti-ARA55 / HIC-5 Antibody (N-Terminus) IHC-plus™ LS-B3528
Rabbit Polyclonal (IgG) to Human ARA55 / HIC-5
Human, Mouse, Rat, Dog, Rabbit
IHC - Paraffin, Western blot
Unconjugated
Immunohistochemistry Image
Anti-ARA55 / HIC-5 Antibody (aa256-461, Biotin) LS-C297039
Rabbit Polyclonal (IgG) to Rat ARA55 / HIC-5
Rat
Western blot, ELISA
Biotin Conjugated
Western blot Image

100% Guaranteed 100% Guaranteed
Rabbit Polyclonal to Human ARA55 / HIC-5
Human, Monkey, Mouse, Rat, Bovine, Goat, Guinea pig, Horse, Rabbit
IHC - Paraffin, Western blot (applications tested for the base form of this product only)
Unconjugated

Details

Human ARA55 / HIC-5
Rabbit
Human, Monkey, Mouse, Rat, Bovine, Goat, Guinea pig, Horse, Rabbit (tested or 100% immunogen sequence identity)
Bat, Dog (at least 90% immunogen sequence identity)
Polyclonal
Unconjugated. Also available conjugated with Biotin, FITC, HRP.
Protein A purified
Unmodified

Applications

  • IHC - Paraffin (5 µg/ml)
  • Western blot (0.2 - 1 µg/ml)
  • (applications tested for the base form of this product only)

Specificity and Use

ARA55 / HIC-5 antibody was raised against synthetic peptide located between aa179-228 of human TGFB1I1 (B2R8D5, NP_057011). Percent identity by BLAST analysis: Human, Chimpanzee, Gorilla, Gibbon, Monkey, Galago, Marmoset, Mouse, Rat, Elephant, Bovine, Rabbit, Horse, Guinea pig (100%); Panda, Dog, Bat, Opossum (92%); Goat (85%).
LS-E11816 - Lyophilized - 100 µg - $145.00
aa179-228
Human TGFB1I1 / HIC-5
Immunohistochemistry: LS-B1004 was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for LS-B1004 was determined to be 5 ug/ml.Antibody tested in peptide-based ELISA.

Packaging

Lyophilized from PBS with 2% sucrose
50 µl After adding water, will consist of PBS buffer with 2% sucrose
Long term: -20°C, the use of 50% glycerol is recommended if storing aliquots in -20°C for long term use (up to 1 year); Short term (less than one week): +4°C. Avoid repeat freeze-thaw cycles.
For research use only.

About ARA55 / HIC-5

O43294 NM_015927 NP_057011.2

TGFB1I1 Antibody, ARA55 Antibody, HIC5 Antibody, HIC-5 Antibody, TSC-5 Antibody

Functions as a molecular adapter coordinating multiple protein-protein interactions at the focal adhesion complex and in the nucleus. Links various intracellular signaling modules to plasma membrane receptors and regulates the Wnt and TGFB signaling pathways. May also regulate SLC6A3 and SLC6A4 targeting to the plasma membrane hence regulating their activity.

Immunohistochemistry

Immunohistochemistry
Anti-TGFB1I1 / HIC-5 antibody IHC of human prostate. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B1004 concentration 5 ug/ml. This image was taken for the unconjugated form of this product. Other forms have not been tested.

Western blot

Western blot
TGFB1I1 / HIC-5 antibody LS-B1004 Western blot of Fetal lung lysate. This image was taken for the unconjugated form of this product. Other forms have not been tested.

Western blot

Western blot
Fetal Kidney Lysate. This image was taken for the unconjugated form of this product. Other forms have not been tested.
 

Requested From: United States
Date Requested: 12/9/2016

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