Work with LifeSpan to design a custom immunohistochemistry to address your specific biological question. Outsource the entire localization process without having to
worry about finding and characterizing target specific antibodies, sourcing and validating difficult-to-find tissues, and having the ability to interpret the resulting
immunostaining in relation to complex human pathologies.
TCR Screening Services
Test your therapeutic antibodies in immunohistochemistry against a broad panel of normal frozen human tissue types in order to determine potential unintended binding.
Our non-GLP TCR services are designed on the FDA recommendation outlined in their "Points to Consider in the Manufacture and Testing of Monoclonal Antibody Products for Human Use".
The apolipoprotein B mRNA editing protein (APOBEC1) is involved in the production of apolipoprotein B (apoB-)48 from apoB-100. APOBEC1 gene spans 18 kb and contains 5 exons, all of which are translated. The use of an alternative splice acceptor results in an alternative form APOBEC1 mRNA, which misses exon 2 causing a frameshift that produces a novel 36-amino acid peptide in which only the first 6-amino acids are shared with APOBEC1.
Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. BC = breast carcinoma; HC = hepatocarcinoma.
The anti-Apobec1 N-term antibody is used in Western blot to detect Apobec in mouse small intestine tissue lysate.
Western blot of anti-Apobec1 Antibody in 293 cell line lysates (35 ug/lane). Apobec1(arrow) was detected using the purified antibody.
Requested From: United States
Date Requested: 12/4/2016