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Anti-API5 Antibody (clone 1C2) LS-C105065

Catalog Size Price
LS-C105065-100 100 µg Unavailable

Related Products

39 API5 Antibodies

Most Popular API5 Antibodies

Anti-API5 Antibody (aa478-492) LS-C121355
Rabbit Polyclonal (IgG) to Human API5
Human, Monkey, Mouse, Rat, Bovine, Dog, Hamster, Horse, Pig, Rabbit, Chicken
IHC, IHC - Paraffin, Western blot
Unconjugated
Anti-API5 Antibody (aa2-360, Biotin) LS-C688269
Rabbit Polyclonal (IgG) to Human API5
Human
IHC, Western blot
Biotin Conjugated
Western blot of API5 antibody.
Anti-API5 Antibody (aa2-360, FITC) LS-C695199
Rabbit Polyclonal (IgG) to Human API5
Human
IHC, Western blot
FITC Conjugated
Western blot of API5 antibody.
Anti-API5 Antibody (aa2-360, Cy3) LS-C703943
Rabbit Polyclonal (IgG) to Human API5
Human
IHC, Western blot
Cy3 Conjugated
Western blot of API5 antibody.
Anti-API5 Antibody (aa2-360, HRP) LS-C712968
Rabbit Polyclonal (IgG) to Human API5
Human
IHC, Western blot
HRP Conjugated
Western blot of API5 antibody.

100% Guaranteed
Mouse Monoclonal [clone 1C2] (IgG2b,k) to Human API5
Human
Immunofluorescence, Western blot, ELISA
Unconjugated

Details

Human API5
Mouse
Human (tested or 100% immunogen sequence identity)
IgG2b,k Monoclonal [1C2]
Unconjugated
Purified from ascites by Protein A
Unmodified

Applications

  • Immunofluorescence (10 µg/ml)
  • Western blot
  • ELISA

  • IHC - Paraffin

Specificity and Use

API5 antibody was raised against aPI5 (AAH17709, 400 a.a. ~ 504 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa.
Western Blot (Cell lysate) - positive control Hela NE. Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections, 3 ug/ml) - positive control human small Intestine. Immunofluorescence (10 ug/ml) - positive control HeLa cells. Sandwich ELISA (Recombinant protein).

Packaging

PBS, pH 7.4
Store at -20°C. Aliquot to avoid freeze/thaw cycles.
For research use only.

About API5

Q9BZZ5 NM_006595 NP_006586.1

API5 Antibody, AAC11 Antibody, AAC-11 Antibody, Antiapoptosis clone 11 protein Antibody, API-5 Antibody, Apoptosis inhibitor 5 Antibody, FIF Antibody, API5L1 Antibody, Protein XAGL Antibody

Antiapoptotic factor that may have a role in protein assembly. Negatively regulates ACIN1. By binding to ACIN1, it suppresses ACIN1 cleavage from CASP3 and ACIN1-mediated DNA fragmentation. Also known to efficiently suppress E2F1-induced apoptosis. Its depletion enhances the cytotoxic action of the chemotherapeutic drugs.

Immunofluorescence

Immunofluorescence of monoclonal antibody to API5 on HeLa cell (antibody concentration 10 ug/ml).
Immunofluorescence of monoclonal antibody to API5 on HeLa cell (antibody concentration 10 ug/ml).

Western blot

API5 monoclonal antibody (M01), clone 1C2 Western blot of API5 expression in HeLa NE.
API5 monoclonal antibody (M01), clone 1C2 Western blot of API5 expression in HeLa NE.

Enzyme-Linked Immunosorbent Assay

Detection limit for recombinant GST tagged API5 is approximately 0.1 ng/ml as a capture antibody.
Detection limit for recombinant GST tagged API5 is approximately 0.1 ng/ml as a capture antibody.

Requested From: 
Date Requested: 5/24/2018

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