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Anti-API5 Antibody (clone 1C2) LS-C105065

Ordering

Wt. Vol. Conc. Price
100 µg - - $355
Inquire for larger quantities

LSBio (Direct) LSBio (Direct)
206-374-1102
866-206-6909
Orders@LSBio.com
 

Most Popular API5 Antibodies

Anti-API5 Antibody (aa478-492) LS-C121355
Rabbit Polyclonal (IgG) to Human API5
Human, Monkey, Mouse, Rat, Bovine, Dog, Hamster, Horse, Pig, Rabbit, Chicken
IHC - Paraffin, Western blot
Unconjugated
Anti-API5 Antibody (aa421-470) IHC-plus™ LS-B8532
Rabbit Polyclonal (IgG) to Human API5
Human, Mouse
IHC - Paraffin, Western blot, ELISA
Unconjugated
Immunohistochemistry Image
Anti-API5 Antibody (aa482-510, AP) LS-C251513
Rabbit Polyclonal (IgG) to Human API5
Human
Western blot, ELISA
AP Conjugated

100% Guaranteed 100% Guaranteed
Mouse Monoclonal [clone 1C2] (IgG2b,k) to Human API5
Human
Immunofluorescence, Western blot, ELISA
Unconjugated

Details

Human API5
Mouse
Human (tested or 100% immunogen sequence identity)
IgG2b,k Monoclonal [1C2]
Unconjugated
Protein A purified
Unmodified

Applications

  • Immunofluorescence (10 µg/ml)
  • Western blot
  • ELISA

  • IHC - Paraffin

Specificity and Use

API5 antibody was raised against aPI5 (AAH17709, 400 a.a. ~ 504 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa.
Western Blot (Cell lysate) - positive control Hela NE. Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections, 3 ug/ml) - positive control human small Intestine. Immunofluorescence (10 ug/ml) - positive control HeLa cells. Sandwich ELISA (Recombinant protein).

Packaging

PBS, pH 7.2
Store at -20°C. Aliquot to avoid freeze/thaw cycles.
For research use only.

About API5

Q9BZZ5 NM_006595 NP_006586.1

API5 Antibody, AAC11 Antibody, AAC-11 Antibody, Antiapoptosis clone 11 protein Antibody, API-5 Antibody, Apoptosis inhibitor 5 Antibody, FIF Antibody, API5L1 Antibody, Protein XAGL Antibody

Antiapoptotic factor that may have a role in protein assembly. Negatively regulates ACIN1. By binding to ACIN1, it suppresses ACIN1 cleavage from CASP3 and ACIN1-mediated DNA fragmentation. Also known to efficiently suppress E2F1-induced apoptosis. Its depletion enhances the cytotoxic action of the chemotherapeutic drugs.

Immunofluorescence

Immunofluorescence
Immunofluorescence of monoclonal antibody to API5 on HeLa cell (antibody concentration 10 ug/ml).

Western blot

Western blot
API5 monoclonal antibody (M01), clone 1C2 Western blot of API5 expression in HeLa NE.

Enzyme-Linked Immunosorbent Assay

Enzyme-Linked Immunosorbent Assay
Detection limit for recombinant GST tagged API5 is approximately 0.1 ng/ml as a capture antibody.
 

Requested From: United States
Date Requested: 12/8/2016

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