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Anti-APC6 / CDC16 Antibody (phospho-Thr580) IHC-plus™ LS-B337

Note: This antibody replaces LS-C18969, LS-C69099, LS-C56899

Ordering

Wt. Vol. Conc. Price
50 µg - 0.97 mg/ml $395
Inquire for larger quantities

LSBio (Direct) LSBio (Direct)
206-374-1102
866-206-6909
Orders@LSBio.com
 

Most Popular APC6 / CDC16 Antibodies

Anti-APC6 / CDC16 Antibody (phospho-Thr580) IHC-plus™ LS-B337
Rabbit Polyclonal to Human APC6 / CDC16
Human
IHC - Paraffin, Immunofluorescence, Western blot, ELISA
Unconjugated
Immunohistochemistry Image
Anti-APC6 / CDC16 Antibody (N-Terminus) IHC-plus™ LS-B4645
Rabbit Polyclonal to Human APC6 / CDC16
Human, Mouse
IHC - Paraffin, Western blot, ELISA
Unconjugated
Immunohistochemistry Image
Anti-APC6 / CDC16 Antibody (aa181-230) IHC-plus™ LS-B13329
Rabbit Polyclonal (IgG) to Human APC6 / CDC16
Human, Mouse, Rat
IHC - Paraffin, Western blot, ELISA
Unconjugated
Immunohistochemistry - Paraffin Image

100% Guaranteed 100% Guaranteed
Rabbit Polyclonal to Human APC6 / CDC16
Human
IHC - Paraffin, Immunofluorescence, Western blot, ELISA
Unconjugated

Details

Human APC6 / CDC16
Rabbit
Human (tested or 100% immunogen sequence identity)
Polyclonal
Unconjugated
Immunoaffinity purified
Unmodified

Applications

  • IHC - Paraffin (5 µg/ml)
  • Immunofluorescence
  • Western blot (1:500 - 1:3000)
  • ELISA (1:10000)

Specificity and Use

APC6 / CDC16 antibody was raised against synthetic peptide from human CDC16 / Apc6.
pThr580
Amino acids 575-588 of Human Apc6 protein.
Immunohistochemistry: LS-B337 was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for LS-B337 was determined to be 5 ug/ml.

Packaging

0.02 M potassium phosphate, 0.15 M sodium chloride, pH 7.2, 0.01% sodium azide.
Long term: -20°C; Short term: +4°C. Avoid repeat freeze-thaw cycles.
For research use only.

About APC6 / CDC16

Q13042 NM_003903 NP_003894.3

CDC16 Antibody, ANAPC6 Antibody, APC6 Antibody, CDC16 homolog Antibody, Cyclosome subunit 6 Antibody, CDC16Hs Antibody, CUT9 Antibody

Component of the anaphase promoting complex/cyclosome (APC/C), a cell cycle-regulated E3 ubiquitin ligase that controls progression through mitosis and the G1 phase of the cell cycle. The APC/C complex acts by mediating ubiquitination and subsequent degradation of target proteins: it mainly mediates the formation of 'Lys-11'-linked polyubiquitin chains and, to a lower extent, the formation of 'Lys-48'- and 'Lys-63'-linked polyubiquitin chains.

Immunohistochemistry

Immunohistochemistry
Anti-CDC16 / Apc6 antibody IHC of human lung, respiratory epithelium. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B337 concentration 5 ug/ml.

Western blot

Western blot
Anti-APC6 pT580 Antibody - Western Blot. Western blot of Affinity Purified anti-APC6 pT580 antibody shows detection of a band ~72 kD corresponding to phosphorylated human APC6 (arrowhead lane 1). Lane 1 - nocodazole treated HeLa whole cell lysate. Lane 2 - Reactivity is not seen in lysates from asynchronous HeLa whole cell cultures. Each lane contains approximately 35 ug of lysates, separated by 4-20% SDS-PAGE Tris-HEPES and then transferred to nitrocellulose. After blocking the membrane was probed with the primary antibody diluted to 1:1000 overnight at 4C followed by washes and reaction with a 1:10000 dilution of IRDye800 conjugated Gt-a-Rabbit IgG [H&L] MX ( for 45 min at room temperature. IRDye800 fluorescence image was captured using the Odyssey Infrared Imaging System developed by LI-COR. IRDye is a trademark of LI-COR, Inc. Other detection systems will yield similar results.

Requested From: United States
Date Requested: 12/5/2016

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