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Work with LifeSpan to design a custom immunohistochemistry to address your specific biological question. Outsource the entire localization process without having to worry about finding and characterizing target specific antibodies, sourcing and validating difficult-to-find tissues, and having the ability to interpret the resulting immunostaining in relation to complex human pathologies.

Test your therapeutic antibodies in immunohistochemistry against a broad panel of normal frozen human tissue types in order to determine potential unintended binding. Our non-GLP TCR services are designed on the FDA recommendation outlined in their "Points to Consider in the Manufacture and Testing of Monoclonal Antibody Products for Human Use".

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ALOX5AP / FLAP Antibody (aa86‑117) LS‑C99454 Best Seller
FLAP antibody LS-C99454 is an unconjugated rabbit polyclonal antibody to human FLAP (ALOX5AP). Validated for IHC and WB.
Catalog
Size
Price
LS-C99454-400
400 µl
Unavailable

Popular ALOX5AP / FLAP Products

Anti-ALOX5AP / FLAP antibody IHC of human lung. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B4933 concentration 3.75 ug/ml.
Species: Human, Monkey
Applications: IHC, IHC - Paraffin, Western blot, Peptide Enzyme-Linked Immunosorbent Assay
Anti-ALOX5AP / FLAP antibody IHC of human tonsil. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B4971 concentration 3.75 ug/ml.
Species: Human
Applications: IHC, IHC - Paraffin, Peptide Enzyme-Linked Immunosorbent Assay
Antibody
Species: Human
Applications: ELISA
Biotinylated antibody (0.3µg/ml) staining of U937 lysate (35µg protein in RIPA buffer), exactly mirroring its parental non-biotinylated product. Primary incubation was 1 hour. Detected by chemiluminescence, using streptavidin-HRP and using NAP blocker as a
Species: Human
Applications: IHC, Western blot, Peptide Enzyme-Linked Immunosorbent Assay

Product Description

FLAP antibody LS-C99454 is an unconjugated rabbit polyclonal antibody to human FLAP (ALOX5AP). Validated for IHC and WB.
About ALOX5AP / FLAP
ALOX5AP / FLAP is a protein which, with 5-lipoxygenase, is required for leukotriene synthesis. Leukotrienes are arachidonic acid metabolites which have been implicated in various types of inflammatory responses, including asthma, arthritis and psoriasis. This protein localizes to the plasma membrane. Inhibitors of its function impede translocation of 5-lipoxygenase from the cytoplasm to the cell membrane and inhibit 5-lipoxygenase activation. P20292 NM_001629 NP_001620.2

ALOX5AP Antibody, FLAP Antibody, MK-886-binding protein Antibody


Specifications

Target
Human ALOX5AP / FLAP
Host
Rabbit
Reactivity
Human (tested or 100% immunogen sequence identity)
Predicted
Monkey, Mouse, Rat, Pig, Rabbit, Cow (at least 90% immunogen sequence identity)
Clonality
Polyclonal
Conjugations
Unconjugated
Purification
Ammonium sulfate precipitation
Modifications
Unmodified
Applications
  • IHC
  • IHC - Paraffin (1:10 - 1:50)
  • Western blot (1:1000)
Performing IHC? See our complete line of Immunohistochemistry Reagents including antigen retrieval solutions, blocking agents ABC Detection Kits and polymers, biotinylated secondary antibodies, substrates and more.
Blocking Peptide
LS-E9356 - Lyophilized - 100 µg - $145.00
Immunizing peptide used to generate LS-C99454. Useful for pre-absorption and neutralization of the antibody's antigen binding site.
Epitope
aa86-117
Specificity
This ALOX5AP antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 86-117 amino acids from the Central region of human ALOX5AP.
Presentation
PBS, 0.09% sodium azide
Storage
Maintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C.
Restrictions
For research use only.
Guarantee
This antibody carries the LSBio 100% Guarantee.

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Images


Immunohistochemistry

Formalin-fixed and paraffin-embedded human lung carcinoma tissue reacted with ALOX5AP antibody , which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.
Formalin-fixed and paraffin-embedded human lung carcinoma tissue reacted with ALOX5AP antibody , which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.

Western blot

Western blot of anti-ALOX5AP Antibody in K562 cell line lysates (35 ug/lane). ALOX5AP(arrow) was detected using the purified antibody.
Western blot of anti-ALOX5AP Antibody in K562 cell line lysates (35 ug/lane). ALOX5AP(arrow) was detected using the purified antibody.

Immunohistochemistry

Formalin-fixed and paraffin-embedded human lung carcinoma tissue reacted with ALOX5AP antibody , which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.
Formalin-fixed and paraffin-embedded human lung carcinoma tissue reacted with ALOX5AP antibody , which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.

Western blot

Western blot of anti-ALOX5AP Antibody in K562 cell line lysates (35 ug/lane). ALOX5AP(arrow) was detected using the purified antibody.
Western blot of anti-ALOX5AP Antibody in K562 cell line lysates (35 ug/lane). ALOX5AP(arrow) was detected using the purified antibody.

Immunohistochemistry

Formalin-fixed and paraffin-embedded human lung carcinoma tissue reacted with ALOX5AP antibody , which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.
Formalin-fixed and paraffin-embedded human lung carcinoma tissue reacted with ALOX5AP antibody , which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.

Western blot

Western blot of anti-ALOX5AP Antibody in K562 cell line lysates (35 ug/lane). ALOX5AP(arrow) was detected using the purified antibody.
Western blot of anti-ALOX5AP Antibody in K562 cell line lysates (35 ug/lane). ALOX5AP(arrow) was detected using the purified antibody.

Immunohistochemistry

Formalin-fixed and paraffin-embedded human lung carcinoma tissue reacted with ALOX5AP antibody , which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.
Formalin-fixed and paraffin-embedded human lung carcinoma tissue reacted with ALOX5AP antibody , which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.

Western blot

Western blot of anti-ALOX5AP Antibody in K562 cell line lysates (35 ug/lane). ALOX5AP(arrow) was detected using the purified antibody.
Western blot of anti-ALOX5AP Antibody in K562 cell line lysates (35 ug/lane). ALOX5AP(arrow) was detected using the purified antibody.


Requested From: 
Date Requested: 6/23/2018

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