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Anti-ALDH2 Antibody (aa52-81) IHC-plus™ LS-B3390

Note: This antibody replaces LS-C98146

Ordering

Wt. Vol. Conc. Price
- 200 µl 0.3 mg/ml $395
Inquire for larger quantities

LSBio (Direct) LSBio (Direct)
206-374-1102
866-206-6909
Orders@LSBio.com
 

Most Popular ALDH2 Antibodies

Anti-ALDH2 Antibody (aa52-81) IHC-plus™ LS-B3390
Rabbit Polyclonal to Human ALDH2
Human
IHC - Paraffin, Immunofluorescence, Western blot, Flow Cytometry
Unconjugated
Immunohistochemistry Image
Anti-ALDH2 Antibody (Internal, clone EPR4493) IHC-plus™ LS-B7921
Rabbit Monoclonal [clone EPR4493] (IgG) to Human ALDH2
Human, Mouse, Rat
IHC - Paraffin, ICC, Western blot, Immunoprecipitation
Unconjugated
Immunohistochemistry Image
Anti-ALDH2 Antibody (aa18-33) LS-C313509
Rabbit Polyclonal (IgG) to Human ALDH2
Human, Monkey, Rabbit
IHC - Paraffin, Western blot
Unconjugated
Immunohistochemistry Image

100% Guaranteed 100% Guaranteed
Rabbit Polyclonal to Human ALDH2
Human
IHC - Paraffin, Immunofluorescence, Western blot, Flow Cytometry
Unconjugated

Details

Human ALDH2
Rabbit
Human (tested or 100% immunogen sequence identity)
Polyclonal
Unconjugated
Immunoaffinity purified
Unmodified

Applications

  • IHC - Paraffin (10 µg/ml)
  • Immunofluorescence (1:10 - 1:50)
  • Western blot (1:1000)
  • Flow Cytometry

Specificity and Use

ALDH2 antibody was raised against kLH-conjugated synthetic peptide between amino acids 52-81 from N-terminal region of human ALDH2.
LS-E3177 - Lyophilized - 100 µg - $145.00
aa52-81
Immunohistochemistry: LS-B3390 was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for LS-B3390 was determined to be 10 ug/ml.

Packaging

PBS, 0.09% sodium azide
Maintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C.
For research use only.

About ALDH2

P05091 NM_000690 NP_000681.2

ALDH2 Antibody, Acetaldehyde dehydrogenase 2 Antibody, ALDH-E2 Antibody, ALDH class 2 Antibody, ALDHI Antibody, ALDM Antibody, Liver mitochondrial ALDH Antibody

This protein belongs to the aldehyde dehydrogenase family of proteins. Aldehyde dehydrogenase is the second enzyme of the major oxidative pathway of alcohol metabolism. Two major liver isoforms of aldehyde dehydrogenase, cytosolic and mitochondrial, can be distinguished by their electrophoretic mobilities, kinetic properties, and subcellular localizations. Most Caucasians have two major isozymes, while approximately 50% of Orientals have the cytosolic isozyme but not the mitochondrial isozyme.

Immunohistochemistry

Immunohistochemistry
Anti-ALDH2 antibody IHC of human liver. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B3390 concentration 10 ug/ml.

Immunofluorescence

Immunofluorescence
Fluorescent confocal image of A549 cell stained with ALDH2 Antibody. A549 cells were fixed with 4% PFA (20 min), permeabilized with Triton X-100 (0.1%, 10 min), then incubated with ALDH2 primary antibody (1:25, 1 h at 37°C). For secondary antibody, Alexa Fluor 488 conjugated donkey anti-rabbit antibody (green) was used (1:400, 50 min at 37°C). Cytoplasmic actin was counterstained with Alexa Fluor 555 (red) conjugated Phalloidin (7units/ml, 1 h at 37°C). Nuclei were counterstained with DAPI (blue) (10 ug/ml, 10 min). ALDH2 immunoreactivity is localized to Mitochondrion significantly.

Western blot

Western blot
ALDH2 Antibody western blot of A549,HepG2,NCI-H292 cell line,mouse liver,lung and rat liver tissue lysates (35 ug/lane). The ALDH2 antibody detected the ALDH2 protein (arrow).
 

Requested From: United States
Date Requested: 12/6/2016

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