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Anti-ALAS1 Antibody (aa480-530) IHC-plus™ LS-B2013

Note: This antibody replaces LS-C622, LS-C68824

Ordering

Wt. Vol. Conc. Price
50 µg - 0.5 mg/ml $395
Inquire for larger quantities

LSBio (Direct) LSBio (Direct)
206-374-1102
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Most Popular ALAS1 Antibodies

Anti-ALAS1 Antibody (aa480-530) IHC-plus™ LS-B2013
Rabbit Polyclonal (IgG) to Human ALAS1
Human
IHC - Paraffin, Western blot
Unconjugated
Immunohistochemistry Image
Anti-ALAS1 Antibody LS-C185224
Rabbit Polyclonal (IgG)
Unconjugated
Anti-ALAS1 Antibody (aa146-195) IHC-plus™ LS-B12725
Rabbit Polyclonal (IgG) to Human ALAS1
Human, Monkey, Mouse, Rat, Bovine, Dog, Guinea pig, Horse
IHC - Paraffin, Western blot
Unconjugated
Immunohistochemistry - Paraffin Image

100% Guaranteed 100% Guaranteed Publications Publications (1)
Rabbit Polyclonal (IgG) to Human ALAS1
Human
IHC - Paraffin, Western blot
Unconjugated

Details

Human ALAS1
Rabbit
Human (tested or 100% immunogen sequence identity)
Dog, Chicken (at least 90% immunogen sequence identity)
IgG Polyclonal
Unconjugated
Protein G purified
Unmodified

Applications

  • IHC - Paraffin (5 µg/ml)
  • Western blot (1 - 3 µg/ml)

Specificity and Use

ALAS1 antibody was raised against synthetic peptide from human ALAS1.
aa480-530
A portion of amino acids 480-530 of human ALAS-1 was used as the immunogen.
Immunohistochemistry: LS-B2013 was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for LS-B2013 was determined to be 5 ug/ml.

Packaging

PBS, 0.05% BSA, 0.05% sodium azide
Store at 4°C for short term applications. For long term storage, aliquot and store at -20°C.
For research use only.

Publications (1)

1
Nuclear receptor 5A (NR5A) family regulates 5-aminolevulinic acid synthase 1 (ALAS1) gene expression in steroidogenic cells. Ju Y, Mizutani T, Imamichi Y, Yazawa T, Matsumura T, Kawabe S, Kanno M, Umezawa A, Kangawa K, Miyamoto K. Endocrinology. 2012 Nov;153:5522-34. [Full Text Article] [PubMed:23024262]

About ALAS1

P13196 NM_000688 NP_000679.1

ALAS1 Antibody, ALAS Antibody, ALAS-H Antibody, ALAS3 Antibody, ALASH Antibody, Delta-ALA synthase 1 Antibody, MIG4 Antibody, Migration-inducing protein 4 Antibody

ALAS1 encodes the mitochondrial enzyme which is catalyzes the rate-limiting step in heme (iron-protoporphyrin) biosynthesis. The enzyme encoded by this gene is the housekeeping enzyme; a separate gene encodes a form of the enzyme that is specific for erythroid tissue. The level of the mature encoded protein is regulated by heme: high levels of heme down-regulate the mature enzyme in mitochondria while low heme levels up-regulate. A pseudogene of this gene is located on chromosome 12.

Immunohistochemistry

Immunohistochemistry
Anti-ALAS1 antibody IHC of human skin. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B2013 concentration 5 ug/ml.

Western blot

Western blot
Western blot of ALAS-1 in HepG2 cell lysate using antibody at 1 ug/ml.

Requested From: United States
Date Requested: 12/6/2016

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