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Anti-AF5Q31 / AFF4 Antibody (clone 2E12) LS-C105131

Catalog Size Price
LS-C105131-100 100 µg Unavailable

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100% Guaranteed
Mouse Monoclonal [clone 2E12] (IgG1,k) to Human AF5Q31 / AFF4
Human
Western blot, ELISA
Unconjugated

Details

Human AF5Q31 / AFF4
Mouse
Human (tested or 100% immunogen sequence identity)
IgG1,k Monoclonal [2E12]
Unconjugated
Purified from ascites by Protein A
Unmodified

Applications

  • Western blot
  • ELISA

  • IHC - Paraffin

Specificity and Use

AF5Q31 / AFF4 antibody was raised against aFF4 (NP_055238, 1 a.a. ~ 109 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa.
Western Blot (Transfected lysate) - positive control transfected 293T cell line. Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections, 1 ug/ml) - positive control human endometrium. Sandwich ELISA (Recombinant protein).

Packaging

PBS, pH 7.2
Store at -20°C. Aliquot to avoid freeze/thaw cycles.
For research use only.

About AF5Q31 / AFF4

Q9UHB7 NM_014423 NP_055238.1

AFF4 Antibody, AF4/FMR2 family member 4 Antibody, AF4/FMR2 family, member 4 Antibody, AF5Q31 Antibody, MCEF Antibody, Protein AF-5q31 Antibody

Key component of the super elongation complex (SEC), a complex required to increase the catalytic rate of RNA polymerase II transcription by suppressing transient pausing by the polymerase at multiple sites along the DNA. In the SEC complex, AFF4 acts as a central scaffold that recruits other factors through direct interactions with ELL proteins (ELL, ELL2 or ELL3) and the P-TEFb complex.

Western blot

Western blot
Western blot of AFF4 expression in transfected 293T cell line by AFF4 monoclonal antibody (M01), clone 2E12.

Enzyme-Linked Immunosorbent Assay

Enzyme-Linked Immunosorbent Assay
Detection limit for recombinant GST tagged AFF4 is approximately 0.03 ng/ml as a capture antibody.

Requested From: 
Date Requested: 10/23/2017

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