(tested or 100% immunogen sequence identity)
Protein A purified
- Western blot
- Flow Cytometry
Acrosin antibody was raised against acid extracts of boar spermatozoa were subjected to hydrophobic chromatography and the pooled fraction with reactivity to N-alpha benzoyl arginine-4-nitroanilide was used for immunization.
Reacts with various forms of porcine acrosin (55, 53, 45 and 35 kD), a typical serine proteinase with trypsin-like specificity. Acrosin is stored in the acrosome of undamaged spermatozoa.
Flow Cytometry: described in Ded et al., 2010. Immunocytochemistry: Application note - Membrane permeabilization (acetone) is essential.
PBS, 15 mM sodium azide, approx., pH 7.4.
Short term 4°C, long term aliquot and store at -20°C, avoid freeze-thaw cycles.
For research use only.
This antibody carries the LSBio 100% Guarantee.