(tested or 100% immunogen sequence identity)
Purified by protein-A affinity chromatography. Greater than 95% by SDS-PAGE.
- Western blot
- Flow Cytometry
Acrosin antibody was raised against acid extracts of boar spermatozoa were subjected to hydrophobic chromatography and the pooled fraction with reactivity to N-alpha benzoylarginine-4-nitroanilide was used for immunization.
The antibody ACR-2 reacts with various forms of porcine acrosin (55, 53, 45 and 35 kDa), a typical serine proteinase with trypsin-like specificity. Acrosin is stored in the acrosome of undamaged spermatozoa.
Flow Cytometry: described in Ded et al., 2010. Immunocytochemistry: Application note - Membrane permeabilization (acetone) is essential.
PBS, pH 7.4, 15 mM Sodium Azide
Store at 2°C to 8°C. Do not freeze.
For research use only.