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Anti-ACOT11 / THEA Antibody (aa19-250, clone J4B2) IHC-plus™ LS-B2961

Note: This antibody replaces LS-C93731

Ordering

Wt. Vol. Conc. Price
50 µg - - $395
Inquire for larger quantities

LSBio (Direct) LSBio (Direct)
206-374-1102
866-206-6909
Orders@LSBio.com
 

Most Popular ACOT11 / THEA Antibodies

Anti-ACOT11 / THEA Antibody (aa19-250, clone J4B2) IHC-plus™ LS-B2961
Mouse Monoclonal [clone J4B2] (IgG2b,k) to Human ACOT11 / THEA
Human
IHC - Paraffin, Western blot, ELISA
Unconjugated
Immunohistochemistry Image
Anti-ACOT11 / THEA Antibody (aa549-575, Biotin) LS-C250689
Rabbit Polyclonal (IgG) to Human ACOT11 / THEA
Human
IHC, Western blot, Flow Cytometry, ELISA
Biotin Conjugated
Anti-ACOT11 / THEA Antibody (aa215-264) IHC-plus™ LS-B11525
Rabbit Polyclonal to Human ACOT11 / THEA
Human, Monkey, Mouse, Rat, Bovine, Dog, Guinea pig, Horse, Rabbit, Chicken, Xenopus
IHC - Paraffin, Western blot
Unconjugated
Immunohistochemistry Image

100% Guaranteed 100% Guaranteed
Mouse Monoclonal [clone J4B2] (IgG2b,k) to Human ACOT11 / THEA
Human
IHC - Paraffin, Western blot, ELISA
Unconjugated

Details

Human ACOT11 / THEA
Mouse
Human (tested or 100% immunogen sequence identity)
IgG2b,k Monoclonal [J4B2]
Unconjugated
Protein G purified
Unmodified

Applications

  • IHC - Paraffin (5 µg/ml)
  • Western blot (1:500 - 1:1000)
  • ELISA

Specificity and Use

ACOT11 / THEA antibody was raised against recombinant human ACOT11 (19-250aa) purified from E. coli.
aa19-250
Immunohistochemistry: LS-B2961 was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for LS-B2961 was determined to be 5 ug/ml.

Packaging

PBS, pH 7.4, 0.1% sodium azide
Long term: -20°C; Short term: +4°C; Avoid freeze-thaw cycles.
For research use only.

About ACOT11 / THEA

Q8WXI4 AK023937 BAB14734.1

ACOT11 Antibody, BFIT2 Antibody, Acyl-CoA thioesterase 11 Antibody, KIAA0707 Antibody, STARD14 Antibody, THEM1 Antibody, START domain containing 14 Antibody, THEA Antibody, BFIT Antibody, BFIT1 Antibody

ACOT11 / THEA is a member of the acyl-CoA thioesterase family which catalyse the conversion of activated fatty acids to the corresponding non-esterified fatty acid and coenzyme A. Expression of a mouse homolog in brown adipose tissue is induced by low temperatures and repressed by warm temperatures. Higher levels of expression of the mouse homolog has been found in obesity-resistant mice compared with obesity-prone mice, suggesting a role of acyl-CoA thioesterase 11 in obesity.

Immunohistochemistry

Immunohistochemistry
Anti-ACOT11 antibody IHC of human spleen. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B2961 concentration 5 ug/ml.

Immunohistochemistry

Immunohistochemistry
Anti-ACOT11 antibody IHC of human small intestine. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B2961 concentration 5 ug/ml.

Western blot

Western blot
Cell lysates of LNCap, Hep3B (30 ug) were resolved by SDS-PAGE, transferred to NC membrane and probed with anti-human ACOT11 (1:1000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.
 

Requested From: United States
Date Requested: 12/6/2016

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