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Anti-ACAA1 Antibody (aa36-85) IHC-plus™ LS-B4015

Note: This antibody replaces LS-C102295
Catalog Size Price
LS-B4015-50 50 µg Unavailable

Related Products

52 ACAA1 Antibodies

Most Popular ACAA1 Antibodies

Anti-ACAA1 Antibody (Biotin) LS-C688009
Rabbit Polyclonal to Human ACAA1
Human, Mouse
IHC, Western blot
Biotin Conjugated
Anti-ACAA1 Antibody (Biotin) LS-C688011
Rabbit Polyclonal to Mouse ACAA1
Mouse, Human
IHC, Western blot
Biotin Conjugated
Western Blot; Sample: Recombinant protein.
Anti-ACAA1 Antibody (FITC) LS-C694919
Rabbit Polyclonal to Human ACAA1
Human, Mouse
IHC, Western blot
FITC Conjugated
Anti-ACAA1 Antibody (FITC) LS-C694920
Rabbit Polyclonal to Mouse ACAA1
Mouse, Human
IHC, Western blot
FITC Conjugated
Western Blot; Sample: Recombinant protein.
Anti-ACAA1 Antibody (Cy3) LS-C703622
Rabbit Polyclonal to Mouse ACAA1
Mouse, Human
IHC, Western blot
Cy3 Conjugated
Western Blot; Sample: Recombinant protein.

100% Guaranteed
Rabbit Polyclonal (IgG) to Human ACAA1
Human, Monkey, Mouse, Rat, Guinea pig, Horse, Rabbit, Zebrafish
IHC, IHC - Paraffin, Western blot (applications tested for the base form of this product only)
Unconjugated

Details

Human ACAA1
Rabbit
Human, Monkey, Mouse, Rat, Guinea pig, Horse, Rabbit, Zebrafish (tested or 100% immunogen sequence identity)
Bovine, Dog, Chicken, Xenopus (at least 90% immunogen sequence identity)
IgG Polyclonal
Unconjugated. Also available conjugated with Biotin, FITC, HRP.
Immunoaffinity purified
Unmodified

Applications

  • IHC
  • IHC - Paraffin (5 µg/ml)
  • Western blot (1 µg/ml)
  • (applications tested for the base form of this product only)

Specificity and Use

ACAA1 antibody was raised against synthetic peptide located between aa36-85 of human ACAA1 (P09110, NP_001598). Percent identity by BLAST analysis: Human, Chimpanzee, Gorilla, Monkey, Galago, Marmoset, Rat, Panda, Rabbit, Horse, Guinea pig (100%); Gibbon, Elephant, Dog, Bovine, Opossum, Zebra finch, Chicken (92%); Lizard, Xenopus, Stickleback (90%); Mouse, Zebrafish (85%).
LS-E17428 - Lyophilized - 100 µg - $145.00
Immunizing peptide used to generate LS-B4015. Useful for pre-absorption and neutralization of the antibody's antigen binding site.
aa36-85
Human ACAA1
Immunohistochemistry: LS-B4015 was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for LS-B4015 was determined to be 5 ug/ml. Western Blot: Suggested dilution at 1µg/ml in 5% skim milk / PBS buffer, and HRP conjugated anti-Rabbit IgG should be diluted in 1: 50,000 - 100,000 as second antibody. ELISA titer in peptide based assay: 1:1562500.

Packaging

Lyophilized from PBS with 2% sucrose
50 µl Distilled water
Long term: -20°C, the use of 50% glycerol is recommended if storing aliquots in -20°C for long term use (up to 1 year); Short term (less than 1 week): 4°C. Avoid freeze-thaw cycles.
For research use only.

About ACAA1

P09110 NM_001607 NP_001598.1

ACAA1 Antibody, Acetyl-CoA acyltransferase 1 Antibody, ACAA Antibody, Acetyl-CoA acyltransferase Antibody, Beta-ketothiolase Antibody, THIO Antibody, PTHIO Antibody

ACAA1 encodes an enzyme operative in the beta-oxidation system of the peroxisomes. Deficiency of this enzyme leads to pseudo-Zellweger syndrome. Alternative splicing results in multiple transcript variants.

Immunohistochemistry

Anti-ACAA1 antibody IHC of human liver. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B4015 concentration 5 ug/ml.  This image was taken for the unconjugated form of this product. Other forms have not been tested.
Anti-ACAA1 antibody IHC of human liver. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B4015 concentration 5 ug/ml. This image was taken for the unconjugated form of this product. Other forms have not been tested.

Immunohistochemistry

Anti-ACAA1 antibody IHC of human colon. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B4015 concentration 5 ug/ml.  This image was taken for the unconjugated form of this product. Other forms have not been tested.
Anti-ACAA1 antibody IHC of human colon. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B4015 concentration 5 ug/ml. This image was taken for the unconjugated form of this product. Other forms have not been tested.

Western blot

Western analysis of fetal brain lysate.  This image was taken for the unconjugated form of this product. Other forms have not been tested.
Western analysis of fetal brain lysate. This image was taken for the unconjugated form of this product. Other forms have not been tested.

Requested From: 
Date Requested: 5/21/2018

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