LSBio
AKT3 Antibody (Internal, clone 25F6.F6.D8, RPE)

Immunohistochemistry of Mouse Monoclonal anti AKT3 unconjugated Antibody in Mouse Embryonic Kidney. Tissue: Mouse Liver. Fixation: FFPE buffered formalin 10% conc. Ag Retrieval: Heat, Citrate pH 6.2. Pressure Cooker. Primary antibody: 2ug/ml 1.5 hour @ room T. Secondary Ab: MOUSE ON MOUSE HRP POLYMER 45 RT.

AKT3 Antibody (Internal, clone 25F6.F6.D8, RPE)

Primary AntibodyLSBio Guarantee
Antibody:Mouse Monoclonal
Applications:Enzyme-Linked Immunosorbent Assay, Flow Cytometry, Immunofluorescence, Immunohistochemistry (general), Western Blot
Reactivity:Human, Mouse, Rat
Format:Lyophilized
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$594.00each
Catalog Number: LS-C744940-50

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Overview

Description
AKT3 antibody LS-C744940 is an RPE-conjugated mouse monoclonal antibody to AKT3 (Internal) from human. It is reactive with human, mouse and rat. Validated for ELISA, Flow, IF, IHC and WB.
Application
ELISA, FC, IF, IHC, WB
Reactivity
Hu, Ms, Rt
Intended Use
Research Use Only
Guarantees
This antibody carries the LSBio 100% Guarantee.
Category
Antibodies > Primary Antibodies > Monoclonal Antibodies
Alternative Names
AKT3 | AKT3/PKBgamma | PRKBG | PKB gamma | Protein kinase Akt-3 | RAC-gamma | MPPH | PKB-GAMMA | PKBG | Protein kinase B gamma | RAC-PK-gamma | STK-2
UniProt Number
Q9Y243
SwissProt
Q9Y243

Target

Target
Human AKT3
Antigen Species
Human
Epitope
The antibody detects both unphosphorylated and phosphorylated forms of the protein. Anti-AKT3 antibody was purified from tissue culture by Protein A chromatography. Cross reactivity with AKT3 from other species has not been determined, however, the sequence of the immunogen shows 100% identity to human, mouse, and rat, therefore, cross reactivity is expected. Cross-reactivity with AKT2 and AKT has not been determined.
Gene Name
AKT3

Antibody

Host Species
Mouse
Clonality
Monoclonal
Clone
25F6.F6.D8
Isotype
IgG1
Light Chain
Kappa Light Chain
Origin Species
Human

Immunogen

Immunogen
Anti-AKT3 Antibody was prepared from tissue culture supernatant by Protein A affinity chromatography using a synthetic peptide corresponding to internal residues of human AKT3 protein.
Immunogen Type
Synthetic Peptide (Linear)

Format

Conjugate/Label
Phycoerythrin
Format
Lyophilized
Formulation
Lyophilized from 10 mg/mL BSA (Protease and Ig free), 0.02 M Potassium Phosphate, pH 7.2, 0.5 M NaCl
Concentration
1 mg/mL
Preservative
No
Purification Method
Protein A Affinity

Storage & Handling

Storage Conditions
Store vial at 4°C prior to restoration. For extended storage aliquot contents and freeze at -20°C or below. Avoid freeze-thaw cycles. Stable for several weeks at 4°C as an undiluted liquid. Dilute only prior to immediate use.
Recommended Storage Buffer
0.02 M Potassium Phosphate|0.5 M NaCl

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To request an SDS/MSDS form for this product, please contact our Technical Support department at: technical@vectorlabs.com.