LSBio
AKT3 Antibody (Internal, clone 25F6.F6.D8, APC)

Flow Cytometry of Mouse anti-AKT3 antibody. Cells: SK-OV3 Cells. Stimulation: none. Primary antibody: Allophycocyanin AKT3 antibody at 1.0 µg/mL for 20 min at 4°C.

AKT3 Antibody (Internal, clone 25F6.F6.D8, APC)

Primary AntibodyLSBio Guarantee
Antibody:Mouse Monoclonal
Applications:Dot Blot, Enzyme-Linked Immunosorbent Assay, Flow Cytometry, Immunofluorescence, Immunohistochemistry (general), Western Blot
Reactivity:Human, Mouse, Rat
Format:Lyophilized
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$594.00each
Catalog Number: LS-C744945-50

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Overview

Description
AKT3 antibody LS-C744945 is an APC-conjugated mouse monoclonal antibody to AKT3 (Internal) from human. It is reactive with human, mouse and rat. Validated for DB, ELISA, Flow, IF, IHC and WB.
Application
DB, ELISA, FC, IF, IHC, WB
Reactivity
Hu, Ms, Rt
Intended Use
RUO
Guarantees
This antibody carries the LSBio 100% Guarantee. ✓
Category
Antibodies > Primary Antibodies > Monoclonal Antibodies
Alternative Names
AKT3 | AKT3/PKBgamma | PRKBG | PKB gamma | Protein kinase Akt-3 | RAC-gamma | MPPH | PKB-GAMMA | PKBG | Protein kinase B gamma | RAC-PK-gamma | STK-2
Recommended Dilution: FC
1 ug/ml
UniProt Number
Q9Y243
NCBI GenBank
NM_005465, NP_005456.1
SwissProt
Q9Y243

Target

Target
Human AKT3
Antigen Species
Hu
Epitope
The antibody detects both unphosphorylated and phosphorylated forms of the protein. Anti-AKT3 antibody was purified from ascites by Protein A chromatography. Cross reactivity with AKT3 from other species has not been determined, however, the sequence of the immunogen shows 100% identity to human, mouse, and rat, therefore, cross reactivity is expected. Cross-reactivity with AKT2 and AKT has not been determined.
Gene Name
AKT3

Antibody

Host Species
Ms
Clonality
Monoclonal
Clone
25F6.F6.D8
Isotype
IgG1
Light Chain
κ
Origin Species
Hu

Immunogen

Immunogen
Anti-AKT3 Antibody was produced in mice by repeated immunizations with a synthetic peptide corresponding to internal residues of human AKT3 protein.
Immunogen Type
SP-Lin

Format

Conjugate/Label
APC
Format
Lyophilized
Formulation
Lyophilized from 10 mg/mL BSA (Protease and Ig free), 0.02 M Potassium Phosphate, pH 7.2, 0.5 M NaCl
Concentration
1 mg/mL
Preservative
No
Purification Method
Prot A

Storage & Handling

Storage Conditions
Store vial at 4°C prior to restoration. For extended storage aliquot contents and freeze at -20°C or below. Avoid freeze-thaw cycles. Stable for several weeks at 4°C as an undiluted liquid. Dilute only prior to immediate use.
Recommended Storage Buffer
0.02 M Potassium Phosphate|0.5 M NaCl

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