LSBio
Polyclonal Rabbit anti-Human AKT1 Antibody (IHC, WB)

Western blot analysis of Rat Liver showing detection of 55.7 kDa AKT1 protein using Rabbit Anti-AKT1 Polyclonal Antibody. Lane 1: Molecular Weight Ladder (MW). Lane 2: Rat Liver. Load: 15 µg. Block: 5% Skim Milk powder in TBST. Primary Antibody: Rabbit Anti-AKT1 Polyclonal Antibody at 1:1000 for 2 hours at RT with shaking. Secondary Antibody: Goat Anti-Rabbit IgG: HRP at 1:5000 for 1 hour at RT. Color Development: ECL solution for 5 min at RT. Predicted/Observed Size: 55.7 kDa.

Polyclonal Rabbit anti-Human AKT1 Antibody (IHC, WB)

Primary AntibodyLSBio Guarantee
Antibody:Rabbit Polyclonal
Applications:Immunohistochemistry (general), Western Blot
Reactivity:Human, Mouse, Rat
Format:Liquid
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$451.00each
Catalog Number: LS-C774923-100

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Overview

Description
AKT1 antibody LS-C774923 is an unconjugated rabbit polyclonal antibody to AKT1 from human. It is reactive with human, mouse and rat. Validated for IHC and WB.
Application
IHC, WB
Reactivity
Hu, Ms, Rt
Intended Use
RUO
Guarantees
This antibody carries the LSBio 100% Guarantee. ✓
Category
Antibodies > Primary Antibodies > Polyclonal Antibodies
Alternative Names
AKT | AKT1 | AKT-1 | C-AKT | Pan-AKT | PKB alpha | PKBalpha | Proto-oncogene c-Akt | Protein kinase B | Rac protein kinase alpha | PRKBA | Protein kinase B alpha | RAC-PK-alpha | PKB | PKB-ALPHA | RAC | RAC-ALPHA
Recommended Dilution: IHC
1:50
Recommended Dilution: WB
1:1000
UniProt Number
P31749
NCBI GenBank
NM_005163, NP_005154.2
SwissProt
P31749

Target

Target
Human AKT1
Antigen Species
Hu
Epitope
Detects 55 kDa.
Gene Name
AKT1

Antibody

Host Species
Rb
Clonality
Polyclonal
Origin Species
Hu

Immunogen

Immunogen
Synthetic peptide from the N-Terminus of Human RAC-alpha serine/threonine-protein kinase (aa. 1-100)
Immunogen Type
SP-Lin

Format

Conjugate/Label
Unconj
Format
Liquid
Formulation
PBS, pH 7.4, 0.09% Sodium Azide, 50% Glycerol
Concentration
1 mg/mL
Preservative
Yes
Purification Method
AP

Storage & Handling

Storage Conditions
Store at -20°C.
Recommended Storage Buffer
PBS

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