Monoclonal Rat anti-Mouse CD90.2 (Mouse) Antibody (clone 30H12, AF647)
Catalog Number: LS-C810655
2 sizes / from $259.00
Antibody: Rat Monoclonal
Applications: Flow Cytometry
Reactivity: Mouse
Format: Liquid
Immunocytochemistry/Immunofluorescence analysis using Mouse Anti-Acrolein Monoclonal Antibody, Clone 2H2. Tissue: Embryonic kidney epithelial cell line (HEK293). Species: Human. Fixation: 5% Formaldehyde for 5 min. Primary Antibody: Mouse Anti-Acrolein Monoclonal Antibody at 1:50 for 30-60 min at RT. Secondary Antibody: Goat Anti-Mouse Alexa Fluor 488 at 1:1500 for 30-60 min at RT. Counterstain: Phalloidin Alexa Fluor 633 F-Actin stain; DAPI (blue) nuclear stain at 1:250, 1:50000 for 30-60 min at RT. Magnification: 20X (2X Zoom). (A,C,E,G) - Untreated. (B,D,F,H) - Cells cultured overnight with 50 µM H2O2. (A,B) DAPI (blue) nuclear stain. (C,D) Phalloidin Alexa Fluor 633 F-Actin stain. (E,F) Acrolein Antibody. (G,H) Composite. Courtesy of: Dr. Robert Burke, University of Victoria.
Monoclonal Mouse anti-All species Acrolein Antibody (clone 2H2, IF, WB)
Primary AntibodyLSBio Guarantee
| Antibody: | Mouse Monoclonal |
| Applications: | Enzyme-Linked Immunosorbent Assay, Flow Cytometry, Immunofluorescence, Western Blot |
| Reactivity: | Pan-species / Cross-reactive |
| Format: | Liquid |
$507.00each
Catalog Number: LS-C777332-100
Catalog Number: LS-C777332
For bulk orders or custom formulations:
Request a QuoteOverview
- Description
- Acrolein antibody LS-C777332 is an unconjugated mouse monoclonal antibody to all species Acrolein. Validated for ELISA, Flow, IF and WB.
- Application
- ELISA, FC, IF, WB
- Reactivity
- Pan
- Intended Use
- RUO
- Guarantees
- This antibody carries the LSBio 100% Guarantee. ✓
- Category
- Antibodies > Primary Antibodies > Monoclonal Antibodies
- Recommended Dilution: ELISA
- 1:1000
- Recommended Dilution: IF/ICC
- 1:50
- Recommended Dilution: WB
- 1:1000
Target
- Target
- All species Acrolein
- Antigen Species
- Pan
- Epitope
- Specific for Acrolein modified proteins. Does not detect free acrolein. Does not cross-react with Crotonaldehyde, Hexanoyl Lysine, 4-Hydroxy-2-hexenal, 4-Hydroxy nonenal, Malondialdehyde, or Methylglyoxal modified proteins.
Antibody
- Host Species
- Ms
- Clonality
- Monoclonal
- Clone
- 2H2
- Isotype
- IgG1
Immunogen
- Immunogen
- Synthetic Acrolein modified Keyhole Limpet Hemocyanin (KLH).
- Immunogen Type
- Synth
Format
- Conjugate/Label
- Unconj
- Format
- Liquid
- Formulation
- PBS, pH 7.4, 0.09% Sodium Azide, 50% Glycerol
- Concentration
- 1 mg/mL
- Preservative
- Yes
- Purification Method
- Prot G
Storage & Handling
- Storage Conditions
- Store at -20°C.
- Recommended Storage Buffer
- PBS
