LSBio
Acrolein Antibody (clone 10A10)

Flow Cytometry analysis using Mouse Anti-Acrolein Monoclonal Antibody, Clone 10A10. Tissue: Neuroblastoma cells (SH-SY5Y). Species: Human. Fixation: 90% Methanol. Primary Antibody: Mouse Anti-Acrolein Monoclonal Antibody at 1:50 for 30 min on ice. Secondary Antibody: Goat Anti-Mouse: PE at 1:100 for 20 min at RT. Isotype Control: Non Specific IgG. Cells were subject to oxidative stress by treating with 250 µM H2O2 for 24 hours.

Acrolein Antibody (clone 10A10)

Primary AntibodyLSBio Guarantee
Antibody:Mouse Monoclonal
Applications:Enzyme-Linked Immunosorbent Assay, Flow Cytometry, Immunofluorescence, Western Blot
Reactivity:Pan-species / Cross-reactive
Format:Liquid
See all All species Acrolein antibodies →
$507.00each
Catalog Number: LS-C775549-100

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Overview

Description
Acrolein antibody LS-C775549 is an unconjugated mouse monoclonal antibody to all species Acrolein. Validated for ELISA, Flow, IF and WB.
Application
ELISA, FC, IF, WB
Reactivity
Pan
Intended Use
RUO
Guarantees
This antibody carries the LSBio 100% Guarantee. ✓
Category
Antibodies > Primary Antibodies > Monoclonal Antibodies
Recommended Dilution: ELISA
1:1000
Recommended Dilution: FC
1:50
Recommended Dilution: IF/ICC
1:50
Recommended Dilution: WB
1:1000

Target

Target
All species Acrolein
Antigen Species
Pan
Epitope
Specific for Acrolein modified proteins. Does not detect free acrolein. Does not cross-react with Crotonaldehyde, Hexanoyl Lysine, 4-Hydroxy-2-hexenal, 4-Hydroxy nonenal, Malondialdehyde, or Methylglyoxal modified proteins.

Antibody

Host Species
Ms
Clonality
Monoclonal
Clone
10A10
Isotype
IgG1

Immunogen

Immunogen
Synthetic Acrolein modified Keyhole Limpet Hemocyanin (KLH).
Immunogen Type
Synth

Format

Conjugate/Label
Unconj
Format
Liquid
Formulation
PBS, pH 7.4, 0.09% Sodium Azide, 50% Glycerol
Concentration
1 mg/mL
Preservative
Yes
Purification Method
Prot G

Storage & Handling

Storage Conditions
Store at -20°C.
Recommended Storage Buffer
PBS

Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at: technical@vectorlabs.com.