LSBio
Polyclonal Rabbit anti-Human ABCB1 / MDR1 / P Glycoprotein Antibody (IHC, WB)

IHC using ABCB1 Antibody at 20 ug/ml against FFPE human kidney. Moderate to strong cytoplasmic and membranous staining seen in this example. Similar staining also observed in other tissues including liver, kidney, and small intestine. The antibody showed minimal background staining and worked well in formalin-fixed tissues.

Polyclonal Rabbit anti-Human ABCB1 / MDR1 / P Glycoprotein Antibody (IHC, WB)

Primary AntibodyLSBio Guarantee
Antibody:Rabbit Polyclonal
Applications:Enzyme-Linked Immunosorbent Assay, Immunohistochemistry (general), Western Blot
Format:Liquid
See all Human ABCB1 / MDR1 / P Glycoprotein antibodies →
$311.00each
Catalog Number: LS-C745304-25

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Overview

Description
P Glycoprotein antibody LS-C745304 is an unconjugated rabbit polyclonal antibody to P Glycoprotein (ABCB1 / MDR1) from human. It is reactive with human, mouse, rat and other species. Validated for ELISA, IHC and WB.
Application
ELISA, IHC, WB
Predicted Reactivity
Dog, Human, Mouse, Non-Human Primate, Rat
Intended Use
Research Use Only
Guarantees
This antibody carries the LSBio 100% Guarantee.
Category
Antibodies > Primary Antibodies > Polyclonal Antibodies
Alternative Names
ABC20 | ABCB1 | Abcb1b | CLCS | Colchicin sensitivity | CD243 | IBD13 | gp170 | MDR1 | Multidrug resistance protein 1 | P glycoprotein | P-glycoprotein 1 | P-GP | CD243 antigen | PGY1
UniProt Number
P08183
SwissProt
P08183
Recommended Dilution
ApplicationDilution
ELISA1:10000 - 1:50000
WB1:200 - 1:2000

Target

Target
Human ABCB1 / MDR1 / P Glycoprotein
Antigen Species
Human
Epitope
A BLAST analysis was used to suggest cross reactivity with ABCB1 protein from human, monkey, dog, mouse, and rat based on 100% homology with the immunizing sequence. Reactivity against homologues from other sources is not known.
Gene Name
ABCB1

Antibody

Host Species
Rabbit
Clonality
Polyclonal
Isotype
IgG
Origin Species
Human

Immunogen

Immunogen
This affinity purified antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic peptide corresponding to an internal region near amino acids 255-280 of human ABCB1 protein.
Immunogen Type
Synthetic Peptide (Linear)

Format

Conjugate/Label
Unconjugated
Format
Liquid
Formulation
0.02 M Potassium Phosphate, pH 7.2, 0.15 M NaCl, 0.01% Sodium Azide
Concentration
1 mg/mL
Volume
25 Microliter
Preservative
Yes
Purification Method
Affinity Purified

Storage & Handling

Storage Conditions
Store vial at -20°C or below prior to opening. Dilute 1:10 to minimize loss. Store the vial at -20°C or below after dilution. Avoid freeze-thaw cycles.
Recommended Storage Buffer
0.02 M Potassium Phosphate|0.15 M NaCl

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To request an SDS/MSDS form for this product, please contact our Technical Support department at: technical@vectorlabs.com.