Human Apolipoprotein H Antibody (IgA) ELISA Kit (Sandwich ELISA)
Catalog Number: LS-F67373-1
1 plate / $591.00
Reactivity: Human
Detection Method Colorimetric
Format: 96-Well Strip Plate
Western Blot analysis of 4-hydroxy-nonenal-BSA Conjugate showing detection of 67 kDa 4-hydroxy-nonenal protein using Mouse Anti-4-hydroxy-nonenal Monoclonal Antibody, Clone 12F7. Lane 1: Molecular Weight Ladder (MW). Lane 2: BSA (0.5 µg). Lane 3: 4-hydroxyl nonenal-BSA (0.5 µg). Lane 4: 4-hydroxy nonenal-BSA (2.0 µg). Lane 5: 4-hydroxy-2-hexenal (0.5 µg). Lane 6: 4-hydroxy-2-hexenal (2.0 µg). Block: 5% Skim Milk in TBST. Primary Antibody: Mouse Anti-4-hydroxy-nonenal Monoclonal Antibody at 1:1000 for 2 hours at RT. Secondary Antibody: Goat Anti-Mouse IgG: HRP at 1:2000 for 60 min at RT. Color Development: ECL solution for 5 min in RT. Predicted/Observed Size: 67 kDa.
4-Hydroxynonenal Antibody (clone 12F7, Atto 390)
Primary AntibodyLSBio Guarantee
| Antibody: | Mouse Monoclonal |
| Applications: | Enzyme-Linked Immunosorbent Assay, Immunofluorescence, Western Blot |
| Reactivity: | Pan-species / Cross-reactive |
| Format: | Liquid |
$546.00each
Catalog Number: LS-C775513-100
Catalog Number: LS-C775513
For bulk orders or custom formulations:
Request a QuoteOverview
- Description
- 4-Hydroxynonenal antibody LS-C775513 is an Atto 390-conjugated mouse monoclonal antibody to all species 4-Hydroxynonenal. Validated for ELISA, IF and WB.
- Application
- ELISA, IF, WB
- Reactivity
- Pan
- Intended Use
- Research Use Only
- Guarantees
- This antibody carries the LSBio 100% Guarantee. ✓
- Category
- Antibodies > Primary Antibodies > Monoclonal Antibodies
- Recommended Dilution: ELISA
- 1:1000
- Recommended Dilution: IF/ICC
- 1:50
- Recommended Dilution: WB
- 1:1000
Target
- Target
- All species 4-Hydroxynonenal
- Antigen Species
- Pan-species / Cross-reactive
- Epitope
- Specific for 4-Hydroxynonenal (4-HNE) modified proteins. Does not detect free 4-Hydroxynonenal. Does not cross-react with 4-Hydroxy-2-hexenal, Acrolein, Crotonaldehyde, Hexanoyl Lysine, Malondialdehyde, or Methylglyoxal modified proteins.
Antibody
- Host Species
- Mouse
- Clonality
- Monoclonal
- Clone
- 12F7
- Isotype
- IgG1
Immunogen
- Immunogen
- Synthetic 4-Hydroxynonenal modified Keyhole Limpet Hemocyanin (KLH).
- Immunogen Type
- Synthetic Immunogen
Format
- Conjugate/Label
- Atto 390
- Format
- Liquid
- Formulation
- PBS, pH 7.4, 0.09% Sodium Azide, 50% Glycerol
- Concentration
- 1 mg/mL
- Preservative
- Yes
- Purification Method
- Protein G Affinity
Storage & Handling
- Storage Conditions
- Store at -20°C. Protect from light.
- Recommended Storage Buffer
- PBS
