LSBio Immunohistochemistry Studies
LifeSpan provides high-quality, confidential, protein localization data through its immunohistochemistry services. Reseachers can outsource the entire localization process without
having to worry about finding and characterizing target specific antibodies, sourcing and validating difficult-to-find tissues, and having the ability to interpret the resulting
immunostaining in relation to complex human pathologies. All studies are conducted under R&D conditions, are confidential, and the results are proprietary to the customer.
All custom protein expression-profiling studies are confidential, are performed on a fee-for-service basis without royalties or milestone payments, and include the following:
Each contract research study begins with a customer consultation to define the study goals, available resources, and the optimal scientific approach. LifeSpan has conducted thousands of localization studies for hundreds of companies worldwide, experience that can be applied to help ensure a successful study.
Antibody selection and validation:
Antibodies can be provided by the client, purchased from a commercial resource or acquired from LifeSpan's antibody catalog
Tissue selection and validation:
Tissue specimens are selected that will best answer the biological question. Normal and diseased, frozen and formalin-fixed, human and non-human tissues can be supplied by the client, acquired from LifeSpan's extensive Tissue Bank
, purchased from commercial vendors, or collected prospectively by LifeSpan for use in the study. Prior to use in any study, all tissues are subjected to rigorous testing to ensure proper fixation, accurate diagnosis, and antigen validation.
Antibodies are initially tested in Phase I to ensure that they are working appropriately before being used to screen the selected test tissues in Phase II. This involves performing a serial dilution of the antibody to establish the optimal staining concentration. Optimizing antigen-retrieval conditions may also be necessary to improve the antibody's signal-to-noise ratio on tissue samples.
During Phase II testing, antibodies are used under the optimized assay conditions to immunolabel the selected tissues of interest. LifeSpan can use single or multiple antibodies to provide co-localization information. LifeSpan has extensive experience in double labeling and can recommend markers for differentiating specific cell types.
The resulting immunolabeled slides are interpreted by LifeSpan's staff of pathologists, who have many years experience interpreting the often complex behavior of antibodies in IHC. The cellular localization profile is then correlated with the normal and/or disease processes being evaluated.
LifeSpan has a flexible report format that allows for customization depending upon the level of interpretation required. The two most common forms are Full Text and Tabular reporting.
Full-Text Reporting: With Full-Text Reporting, LifeSpan's pathologists describe the immunolocalization patterns in detail and provide 3-5 representative images for each tissue sample. This format is useful for studies that require detailed observations and interpretation, such as studies involving CNS tissues, co-localization studies, or those that may involve rare events and minor cell types. Information about each patient sample is also provided.
Full text report on Cardiovascular Disease
Full text report on Neurodegenerative Disease
Full text report with Double-labeling
Full text report on Cancer Tissues
Full text report on Inflammatory Disease
Tabular Reporting with and without Imaging: With Tabular Reporting, LifeSpan's pathologists quantify the signal present in major cell types within each tissue sample using a 0-4 scale and present the results in a table. This format is useful for studies at the initial stages of target characterization, screening studies, or those involving major cell types or in homogeneous tissues. Customers can choose to include selected images for each tissue sample.
Tabular report on Cancer Tissues
Upon the conclusion of each study, LifeSpan will provide a report detailing the assay condition, the antibody dilution specifications, and localization results for each tissue specimen assayed. If available, selected patient information, including the age, sex, cause of death or surgery, is provided with each sample. LifeSpan offers a variety of reporting formats to meet the varied needs of our customers. Reports are delivered via a secure website and can also be delivered on CD.
Standard IHC studies take from 3-12 weeks to complete, depending upon the complexity of the study. LifeSpan strives to complete our customers' work in an expeditious manner.
LifeSpan offers unparalleled quality and competitive pricing for its contract research services. Due to the variable nature of these studies, a custom price is quoted for each study.