Overview

Description

ELISA Blocking Buffer (High Strength) provides a high degree of blocking efficiency and protein stabilization through the use of non-mammalian protein blocking agents and stabilizers. The heterogeneous composition of this unique ELISA blocking buffer efficiently blocks the uncoated regions of the microtiter plate without the use of mammalian protein additives. This buffer is best for assays that will not tolerate conventional mammalian blocking buffers or immunoassays with high background problems. In antigen-down ELISA formats, the use of ELISA Blocking Buffer (High Strength) reduces the risk of endogenous antibodies in the sample reacting with the proteins used to block the plate, making ELISA Blocking Buffer (High Strength) particularly useful when testing human, swine, and bovine sera. ELISA Blocking Buffer (High Strength) heterogenous mixture of nonmammalian blocking agents minimizes non specific binding interactions during the immunoassay process, reducing ELISA background noise and increasing specific signal. In addition, the non-mammalian-based ELISA Blocking Buffer (High Strength) formulation is antigenically foreign to most mammalian immune systems, reducing the chance of cross-reactivity with anti-isotype detection antibodies. • pH: 7.4 at 1X • Contains: Non-Mammalian Protein Blocking Agents • Supplied At: 1X • Storage: 2-8°C This buffer also provides a microhydrated environment to stabilize the dried, coated protein during long-term storage for improved retention of antigen epitope and antibody binding activity. Also important when preparing batches for storage is ELISA Blocking Buffer (High Strength) contains an antimicrobial agent, which allows for stable room temperature blocking and long-term refrigerated storage of dried plates.

Category

Reagents & Buffers

Buffer-Reagent Type

Blocking