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Work with LifeSpan to design a custom immunohistochemistry to address your specific biological question. Outsource the entire localization process without having to worry about finding and characterizing target specific antibodies, sourcing and validating difficult-to-find tissues, and having the ability to interpret the resulting immunostaining in relation to complex human pathologies.

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Test your therapeutic antibodies in immunohistochemistry against a broad panel of normal frozen human tissue types in order to determine potential unintended binding. Our non-GLP TCR services are designed on the FDA recommendation outlined in their "Points to Consider in the Manufacture and Testing of Monoclonal Antibody Products for Human Use".

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Catalog Number Size Price
LS-K775-100 100 asy $665 
MME / CD10 Assay Kit - Inhibition of Neprilysin activity by Thiorphan. IC50 of Thiorphan was calculated to be 4.16 nM.

Neprilysin Inhibitor Screening Kit (Fluorometric) - LS-K775

Neprilysin Inhibitor Screening Kit (Fluorometric) - LS-K775

Available for shipment within the USA only
Description:
Neprilysin (NEP, EC 3.4.24.11), also known as neutral endopeptidase, enkephalinase, CD10, and common acute lymphoblastic leukemia antigen, is a type II zinc-containing transmembrane metalloproteinase. It is able to hydrolyze very important endogenous peptides, such as natriuretic atrial factor, enkephalins, substance P, bradykinin and amyloid beta-peptide. Thus, NEP is a potentially therapeutic target in important pathological conditions such as cardiovascular disease, prostate cancer, Alzheimer's disease and analgesia. NEP has also been used as a biological marker of a type of childhood leukemia called CALLA. NEP is currently a focus of interest in cardiovascular and neurobiological research. NEP inhibitors have shown promising results as potential treatment alternative for some conditions including hypertension, heart failure, renal failure, and improvement of Beta-cell function in obese type 2 diabetes mellitus. The combination of NEP inhibitor and angiotensin II type 1 receptor blockade has been used clinically to treat patients with chronic heart failure. The NEP Inhibitor Screening Kit can be used to screen for potential inhibitors of NEP activity. It utilizes the ability of an active NEP to cleave a synthetic MCA based peptide substrate to release a free fluorophore. The released MCA can be easily quantified using a fluorescence microplate reader. In the presence of an NEP specific inhibitor, the enzyme loses its activity which results in decrease of fluorescence intensity. The assay kit is simple and can be used to identify and characterize NEP inhibitors in a high-throughput format
Price
Catalog Number
$665 
LS-K775-100
100 asy

Available for shipment within the USA only
Toll Free North America
866-819-4732

Overview

Description:
Neprilysin (NEP, EC 3.4.24.11), also known as neutral endopeptidase, enkephalinase, CD10, and common acute lymphoblastic leukemia antigen, is a type II zinc-containing transmembrane metalloproteinase. It is able to hydrolyze very important endogenous peptides, such as natriuretic atrial factor, enkephalins, substance P, bradykinin and amyloid beta-peptide. Thus, NEP is a potentially therapeutic target in important pathological conditions such as cardiovascular disease, prostate cancer, Alzheimer's disease and analgesia. NEP has also been used as a biological marker of a type of childhood leukemia called CALLA. NEP is currently a focus of interest in cardiovascular and neurobiological research. NEP inhibitors have shown promising results as potential treatment alternative for some conditions including hypertension, heart failure, renal failure, and improvement of Beta-cell function in obese type 2 diabetes mellitus. The combination of NEP inhibitor and angiotensin II type 1 receptor blockade has been used clinically to treat patients with chronic heart failure. The NEP Inhibitor Screening Kit can be used to screen for potential inhibitors of NEP activity. It utilizes the ability of an active NEP to cleave a synthetic MCA based peptide substrate to release a free fluorophore. The released MCA can be easily quantified using a fluorescence microplate reader. In the presence of an NEP specific inhibitor, the enzyme loses its activity which results in decrease of fluorescence intensity. The assay kit is simple and can be used to identify and characterize NEP inhibitors in a high-throughput format

Specifications

Name
Neprilysin Inhibitor Screening Kit (Fluorometric)
Type
Inhibitor Screening
Usage
Screening/characterizing/studying Neprilysin inhibitors/activators
Target
MME / CD10
Synonyms
MME | Atriopeptidase | CALLA | CD10 | EPN | Membrane metallo-endopeptidase | NEP | Neprilysin | Neutral endopeptidase | Neutral endopeptidase 24.11 | CD10 antigen | Enkephalinase | Neprylisin | SFE | Skin fibroblast elastase
Detection
Fluorometric (Ex/Em = 320/420 nm)
Species Reactivity
Human
Components
  • NEP Assay Buffer
  • NEP Enzyme (Lyophilized)
  • NEP Substrate (in DMSO)
  • NEP Inhibitor (10 mM Thiorpan)
  • (See Datasheet for specific volumes supplied)
  • Applications
    Spectrophotometry (fluorometric)
    Equipment
    Microplate fluorometer
    Conditions
    Shipped -20°C Dry Ice, Components stored at varying temperatures. See recommended storage details in product insert, 12 months shelf life.
    Documents
    Restrictions
    For research use only. Available for shipment within the USA only
    Guarantee
    This Assay Kit carries the LSBio 100% Guarantee
    Product Features
    • High-throughput compatible
    • Includes Inhibitor Control (Thiorpan)
    • Screens Neprilysin inhibitors/activators
    • Simple and reliable

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    Customer Reviews (0)

    Images

    Assay Result

    MME / CD10 Assay Kit - Inhibition of Neprilysin activity by Thiorphan. IC50 of Thiorphan was calculated to be 4.16 nM.
    Inhibition of Neprilysin activity by Thiorphan. IC50 of Thiorphan was calculated to be 4.16 nM.

    Assay Result

    MME / CD10 Assay Kit - Inhibition of Neprilysin activity by Thiorphan. IC50 of Thiorphan was calculated to be 4.16 nM.
    Inhibition of Neprilysin activity by Thiorphan. IC50 of Thiorphan was calculated to be 4.16 nM.

    Assay Result

    MME / CD10 Assay Kit - Inhibition of Neprilysin activity by Thiorphan. IC50 of Thiorphan was calculated to be 4.16 nM.
    Inhibition of Neprilysin activity by Thiorphan. IC50 of Thiorphan was calculated to be 4.16 nM.

    Assay Result

    MME / CD10 Assay Kit - Inhibition of Neprilysin activity by Thiorphan. IC50 of Thiorphan was calculated to be 4.16 nM.
    Inhibition of Neprilysin activity by Thiorphan. IC50 of Thiorphan was calculated to be 4.16 nM.

    Requested From: United States
    Date Requested: 2/23/2020