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Immunohistochemistry Services

Work with LifeSpan to design a custom immunohistochemistry to address your specific biological question. Outsource the entire localization process without having to worry about finding and characterizing target specific antibodies, sourcing and validating difficult-to-find tissues, and having the ability to interpret the resulting immunostaining in relation to complex human pathologies.

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Test your therapeutic antibodies in immunohistochemistry against a broad panel of normal frozen human tissue types in order to determine potential unintended binding. Our non-GLP TCR services are designed on the FDA recommendation outlined in their "Points to Consider in the Manufacture and Testing of Monoclonal Antibody Products for Human Use".

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Cell Cycle Pathways
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Catalog Number Size Price
LS-K421-100 100 asy $1,100 
Assay Kit

AHCY Inhibitor Screening Kit (Fluorometric) - LS-K421

AHCY Inhibitor Screening Kit (Fluorometric) - LS-K421

Available for shipment within the USA only
Description:
Adenosyl homocysteinase (AHCY) (EC 3.3.1.1) or S-adenosyl homocysteine hydrolase (SAHH); is an enzyme that catalyzes the reversible hydrolysis of S-Adenosyl Homocysteine (SAH) to adenosine and homocysteine. Inhibition of AHCY results in the accumulation of SAH, a product inhibitor of S-adenosyl methionine (SAM)-dependent methyltransferases. AHCY is an important target as an antiviral and anticancer drug. Several characterized SAH inhibitors inhibit some DNA viruses (e.g. Pox viruses) and some negative stranded RNA viruses (e.g.: Marburg virus, Ebola virus & rabies). In The AHCY Inhibitor Screening kit the homocysteine generated from the breakdown of SAH is measured using a Thiol Detecting Reagent resulting in enhanced fluorescence which is measured at Ex/Em = 392/482 nm. The AHCY reaction is reversible and hence Adenosine Deaminase is included in the reaction to ensure that the reaction proceeds towards hydrolysis of SAH. In the presence of AHCY inhibitor, there is a decrease in fluorescence of the Thiol Detecting Reagent.
Price
Catalog Number
$1,100 
LS-K421-100
100 asy

Available for shipment within the USA only
Toll Free North America
866-819-4732

Overview

Description:
Adenosyl homocysteinase (AHCY) (EC 3.3.1.1) or S-adenosyl homocysteine hydrolase (SAHH); is an enzyme that catalyzes the reversible hydrolysis of S-Adenosyl Homocysteine (SAH) to adenosine and homocysteine. Inhibition of AHCY results in the accumulation of SAH, a product inhibitor of S-adenosyl methionine (SAM)-dependent methyltransferases. AHCY is an important target as an antiviral and anticancer drug. Several characterized SAH inhibitors inhibit some DNA viruses (e.g. Pox viruses) and some negative stranded RNA viruses (e.g.: Marburg virus, Ebola virus & rabies). In The AHCY Inhibitor Screening kit the homocysteine generated from the breakdown of SAH is measured using a Thiol Detecting Reagent resulting in enhanced fluorescence which is measured at Ex/Em = 392/482 nm. The AHCY reaction is reversible and hence Adenosine Deaminase is included in the reaction to ensure that the reaction proceeds towards hydrolysis of SAH. In the presence of AHCY inhibitor, there is a decrease in fluorescence of the Thiol Detecting Reagent.

Specifications

Name
AHCY Inhibitor Screening Kit (Fluorometric)
Type
Inhibitor Screening
Usage
Screening/characterizing/studying AHCY inhibitors
Target
SAHH / AHCY
Synonyms
AHCY | Adenosylhomocysteinase | AdoHcyase | SAHH
Detection
Fluorometric (Ex/Em 392/482 nm)
Components
  • AHCY Assay Buffer
  • AHCY Reconstitution Buffer
  • AHCY Enzyme
  • Adenosine Deaminase
  • 3-Deazaneplanocin A (10 µM) (in DMSO)
  • AHCY Substrate (in DMSO)
  • Thiol Detecting Reagent (in DMSO)
  • (See Datasheet for specific volumes supplied)
  • Applications
    Spectrophotometry (fluorometric)
    Equipment
    Microplate fluorometer
    Conditions
    Shipped -20°C Dry Ice, Store at -20°C. Avoid freeze-thaw cycles, 12 months shelf life.
    Documents
    Restrictions
    For research use only. Available for shipment within the USA only
    Guarantee
    This Assay Kit carries the LSBio 100% Guarantee
    Product Features
    • High-throughput suitable
    • Includes Inhibitor Control (3-Deazaneplanocin A)
    • Simple and reliable test to screen AHCY inhibitors

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    Requested From: United States
    Date Requested: 2/19/2020