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Catalog Number	Size	Price
LS-C742479-100	100 µg (0.5 mg/ml)	$503

ROBO3 Antibody - Human Cerebellum: Formalin-Fixed, Paraffin-Embedded (FFPE)

ROBO3 Antibody - Human Cortex: Formalin-Fixed, Paraffin-Embedded (FFPE)

1 of 2

2 of 2

Polyclonal Goat anti‑Mouse ROBO3 Antibody (aa1303‑1316, IHC) LS‑C742479

Name: Anti-ROBO3 Antibody | Goat anti-Mouse Polyclonal IHC,Peptide-ELISA | LSBio
Brand: Lifespan BioSciences
SKU: LS-C742479-100
Price: 503.0000 USD
Availability: InStock

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Antibody:

ROBO3 Goat anti-Mouse Polyclonal (aa1303-1316) Antibody

Application:

IHC-P, Peptide-ELISA

Reactivity:

Mouse

Format:

Unconjugated, Unmodified

$503

LS-C742479-100

Toll Free North America

206-374-1102

For Research Use Only

Specifications Validation Publications Reviews Featured Products Request SDS/MSDS

Overview

Antibody:

ROBO3 Goat anti-Mouse Polyclonal (aa1303-1316) Antibody

Application:

IHC-P, Peptide-ELISA

Reactivity:

Mouse

Format:

Unconjugated, Unmodified

Specifications

Description

ROBO3 antibody LS-C742479 is an unconjugated goat polyclonal antibody to mouse ROBO3 (aa1303-1316). Validated for IHC and Peptide-ELISA.

Target

Mouse ROBO3

Synonyms

See All ROBO3 Antibodies

Host

Goat

Reactivity

Mouse (tested or 100% immunogen sequence identity)

Predicted

Human, Mouse, Rat, Dog, Pig, Cow (at least 90% immunogen sequence identity)

Clonality

Polyclonal

Conjugations

Unconjugated

Purification

ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide

Modifications

Unmodified

Immunogen

KESQGRGRGLEACR

Epitope

aa1303-1316

Applications

IHC - Paraffin (5 µg/µl)
Peptide Enzyme-Linked Immunosorbent Assay

Performing IHC? See our complete line of Immunohistochemistry Reagents including antigen retrieval solutions, blocking agents ABC Detection Kits and polymers, biotinylated secondary antibodies, substrates and more.

Usage

Peptide ELISA: antibody detection limit dilution 1:128000. Western blot: Preliminary experiments gave bands at approx 85kDa in Mouse fetal Brain lysates after 1 ug/ml antibody staining. Please note that currently we cannot find an explanation in the literature for the bands we observe given the calculated size of 150kDa according to Mouse AAD11628.1. Both detected bands were successfully blocked by incubation with the immunizing peptide (and BLAST results with the immunizing peptide sequence did not identify any other proteins to explain the additional bands). We would appreciate any feedback from people in the field - have any results been reported with other antibodies/lysates? Have any further splice variants/modified forms been reported?

Presentation

TBS, pH 7.3, 0.02% Sodium Azide, 0.5% BSA

Storage

Aliquot and store at -20°C. Avoid freeze-thaw cycles.

Restrictions

For research use only. Intended for use by laboratory professionals.

Guarantee

This antibody carries the LSBio 100% Guarantee.

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About ROBO3

Uniprot: Q96MS0 NCBI: NM_022370 NP_071765.2