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Antibodies > RFWD3 > LS-C805356
 
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Catalog Number Size Price
LS-C805356-100 100 µl (1 mg/ml) $379 
LS-C805356-200 200 µl (1 mg/ml) $421 
RFWD3 Antibody - Staining HepG2 cells by IF/ICC. The samples were fixed with PFA and permeabilized in 0.1% Triton X-100, then blocked in 10% serum for 45 min at 25°C. The primary antibody was diluted at 1:200 and incubated with the sample for 1 hour at 37°C. An Alexa Fluor 594 conjugated goat anti-rabbit IgG (H+L) Ab, diluted at 1/600, was used as the secondary antibody.
RFWD3 Antibody - Western blot analysis of extracts of MCF-7 cells using RFWD3 antibody. The lane on the left is treated with the antigen-specific peptide.
RFWD3 Antibody - Staining HepG2 cells by IF/ICC. The samples were fixed with PFA and permeabilized in 0.1% Triton X-100, then blocked in 10% serum for 45 min at 25°C. The primary antibody was diluted at 1:200 and incubated with the sample for 1 hour at 37°C. An Alexa Fluor 594 conjugated goat anti-rabbit IgG (H+L) Ab, diluted at 1/600, was used as the secondary antibody.
RFWD3 Antibody - Western blot analysis of extracts of MCF-7 cells using RFWD3 antibody. The lane on the left is treated with the antigen-specific peptide.
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Polyclonal Rabbit anti‑Human RFWD3 Antibody (IF, WB) LS‑C805356

Polyclonal Rabbit anti‑Human RFWD3 Antibody (IF, WB) LS‑C805356

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Antibody:
RFWD3 Rabbit anti-Human Polyclonal Antibody
Application:
IF, WB, Peptide-ELISA
Reactivity:
Human
Format:
Unconjugated, Unmodified
Price
Catalog Number
$379
LS-C805356-100
Toll Free North America
206-374-1102
For Research Use Only
Specifications Validation Publications Reviews Featured Products Request SDS/MSDS

Overview

Antibody:
RFWD3 Rabbit anti-Human Polyclonal Antibody
Application:
IF, WB, Peptide-ELISA
Reactivity:
Human
Format:
Unconjugated, Unmodified

Specifications

Description
RFWD3 antibody LS-C805356 is an unconjugated rabbit polyclonal antibody to human RFWD3. Validated for IF, Peptide-ELISA and WB.
Target
Human RFWD3
Synonyms
RFWD3 | RING finger protein 201 | RNF201
Host
Rabbit
Reactivity
Human (tested or 100% immunogen sequence identity)
Clonality
IgG Polyclonal
Conjugations
Unconjugated
Purification
Peptide affinity chromatography.
Modifications
Unmodified
Applications
  • Immunofluorescence (1:100 - 1:500)
  • Western blot (1:500 - 1:1000)
  • Peptide Enzyme-Linked Immunosorbent Assay (1:20000 - 1:40000)
Usage
For western blots: incubate membrane with diluted antibody overnight in 5% w/v milk , 1X TBS, 0.1% Tween-20 at 4°C with gentle shaking.
Presentation
PBS, pH 7.4, 0.02% Sodium Azide, 50% Glycerol
Storage
Upon receipt, store at -20°C. Avoid freeze-thaw cycles.
Restrictions
For research use only. Intended for use by laboratory professionals.
Guarantee
This antibody carries the LSBio 100% Guarantee.
LSBio Guarantee
About RFWD3
Q6PCD5 NM_018124 NP_060594.3

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Featured Products

RFWD3 Antibody - Immunohistochemical analysis of RFWD3 staining in human brain formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP-conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with hematoxylin and mounted with DPX.
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Applications: IHC, IHC - Paraffin, Western blot
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Reactivity: Rat
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Reactivity: Human, Mouse, Rat
Range: Positive/Negative
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ADGRE2 / EMR2 Antibody (clone 2A1) LS‑C188288
Species: Human
Applications: IHC - Frozen, Western blot, Immunoprecipitation, Flow Cytometry
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CALML3 Protein
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Tag: His
See all RFWD3 Antibodies

Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at:

Technical.Support@LSBio.com
Requested From: United States
Date Requested: 5/27/2024
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