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Work with LifeSpan to design a custom immunohistochemistry to address your specific biological question. Outsource the entire localization process without having to worry about finding and characterizing target specific antibodies, sourcing and validating difficult-to-find tissues, and having the ability to interpret the resulting immunostaining in relation to complex human pathologies.

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C5AR2 / GPR77 / C5L2 Antibody (C-Terminus, APC) IHC-plus™ LS-A105126

Anti-C5L2 antibody IHC of human brain, cortex. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval.

C5AR2 / GPR77 / C5L2 Antibody (C-Terminus, APC) IHC-plus™ LS-A105126

Antibody:
Rabbit Polyclonal to Human C5AR2 / GPR77 / C5L2
Application:
IHC, IHC-P, ELISA
Reactivity:
Human
Format:
Allophycocyanin, Unmodified
Other formats:
Price
Catalog Number
$650 
LS-A105126-100
Toll Free North America
866-819-4732

Overview

Antibody:
Rabbit Polyclonal to Human C5AR2 / GPR77 / C5L2
Application:
IHC, IHC-P, ELISA
Reactivity:
Human
Format:
Allophycocyanin, Unmodified
Other formats:

Specifications

Description
C5L2 antibody LS-A105126 is an APC-conjugated rabbit polyclonal antibody to human C5L2 (C5AR2 / GPR77). Validated for ELISA and IHC. Tested on 20 paraffin-embedded human tissues.

Target
Human C5AR2 / GPR77 / C5L2
Host
Rabbit
Reactivity
Human (tested or 100% immunogen sequence identity)
Clonality
Polyclonal
Conjugations
Allophycocyanin. Also available Unconjugated or conjugated with AP, Biotin, FITC, HRP, PE.  (This antibody is custom conjugated upon order. Following conjugation, the final antibody concentration will be determined and is typically > 0.5 mg/ml.)
Purification
Immunoaffinity purified
Modifications
Unmodified
Applications
  • IHC
  • IHC - Paraffin
  • ELISA
  • (applications tested for the base form of this product only)
Performing IHC? See our complete line of Immunohistochemistry Reagents including antigen retrieval solutions, blocking agents ABC Detection Kits and polymers, biotinylated secondary antibodies, substrates and more.
Immunogen
Synthetic 20 amino acid peptide from C-terminal cytoplasmic domain of human C5L2. Percent identity with other species by BLAST analysis: Human (100%); Gorilla (95%); Monkey (90%); Marmoset (85%).
Blocking Peptide
LS-E28943 - Liquid - 50 µg - $145.00
Immunizing peptide used to generate LS-A105126. Useful for pre-absorption and neutralization of the antibody's antigen binding site.
Epitope
C-Terminus
Specificity
Human C5L2. BLAST analysis of the peptide immunogen showed no homology with other human proteins.
Usage
Immunohistochemistry: LS-A432 was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for LS-A432 was determined to be 10 ug/ml.
Presentation
PBS, 0.1% Sodium Azide
Storage
Aliquot and store undiluted at -20°C or below for up to 1 year. Can be stored undiluted at 4°C for up to 1 month. Avoid freeze-thaw cycles.
Restrictions
For research use only.
Guarantee
This antibody carries the LSBio 100% Guarantee.
LSBio Guarantee
About C5AR2 / GPR77 / C5L2
Q9P296 NM_018485 NP_060955.1

Validation

Anti-C5L2 antibody IHC of human brain, cortex. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval.
IHC Validated
Anti-C5L2 antibody IHC of human brain, cortex. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval.
Anti-C5L2 antibody IHC of human brain, cortex. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval.

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Requested From: United States
Date Requested: 7/16/2019
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