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Work with LifeSpan to design a custom immunohistochemistry to address your specific biological question. Outsource the entire localization process without having to worry about finding and characterizing target specific antibodies, sourcing and validating difficult-to-find tissues, and having the ability to interpret the resulting immunostaining in relation to complex human pathologies.

Test your therapeutic antibodies in immunohistochemistry against a broad panel of normal frozen human tissue types in order to determine potential unintended binding. Our non-GLP TCR services are designed on the FDA recommendation outlined in their "Points to Consider in the Manufacture and Testing of Monoclonal Antibody Products for Human Use".

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DHFRL1 Antibody (aa71‑120) LS‑C111506
DHFRL1 antibody LS-C111506 is an unconjugated rabbit polyclonal antibody to DHFRL1 from human and monkey. Validated for WB.
Catalog
Size
Price
LS-C111506-50
50 µg
Unavailable

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Product Description

DHFRL1 antibody LS-C111506 is an unconjugated rabbit polyclonal antibody to DHFRL1 from human and monkey. Validated for WB.
About DHFRL1
Key enzyme in folate metabolism. Contributes to the de novo mitochondrial thymidylate biosynthesis pathway. Required to prevent uracil accumulation in mtDNA. Binds its own mRNA and that of DHFR. Q86XF0 NP_789785.1

DHFRL1 Antibody, DHFRP4 Antibody, Dihydrofolate reductase-like 1 Antibody


Specifications

Target
Human DHFRL1
Host
Rabbit
Reactivity
Human, Monkey (tested or 100% immunogen sequence identity)
Predicted
Mouse, Rat, Bovine, Guinea pig, Hamster, Horse, Pig (at least 90% immunogen sequence identity)
Clonality
Polyclonal
Conjugations
Unconjugated. Also available conjugated with Biotin, FITC, HRP.
Purification
Immunoaffinity purified
Modifications
Unmodified
Applications
  • Western blot (0.2 - 1 µg/ml)
  • (applications tested for the base form of this product only)
Immunogen
DHFRL1 antibody was raised against synthetic peptide located between aa71-120 of human DHFRL1 (Q86XF0, NP_789785). Percent identity by BLAST analysis: Human, Monkey, Marmoset (100%); Gorilla, Gibbon, Mouse, Rat, Hamster, Panda, Bovine, Horse, Pig, Guinea pig (92%).
Blocking Peptide
LS-E19256 - Lyophilized - 100 µg - $145.00
Immunizing peptide used to generate LS-C111506. Useful for pre-absorption and neutralization of the antibody's antigen binding site.
Epitope
aa71-120
Specificity
Human DHFRL1
Usage
Western Blot: Suggested dilution at 1 ug/ml in 5% skim milk / PBS buffer, and HRP conjugated anti-Rabbit IgG should be diluted in 1: 50,000 - 100,000 as secondary antibody.
Presentation
Lyophilized from PBS with 2% sucrose
Reconstitution
Distilled water
Storage
Long term: -20°C, the use of 50% glycerol is recommended if storing aliquots in -20°C for long term use (up to 1 year); Short term (less than 1 week): 4°C. Avoid freeze-thaw cycles.
Restrictions
For research use only.
Guarantee
This antibody carries the LSBio 100% Guarantee.

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Images


Western blot

DHFRL1 antibody LS-C111506 Western blot of THP-1 cell lysate.  This image was taken for the unconjugated form of this product. Other forms have not been tested.
DHFRL1 antibody LS-C111506 Western blot of THP-1 cell lysate. This image was taken for the unconjugated form of this product. Other forms have not been tested.

Western blot

DHFRL1 antibody LS-C111506 Western blot of THP-1 cell lysate.  This image was taken for the unconjugated form of this product. Other forms have not been tested.
DHFRL1 antibody LS-C111506 Western blot of THP-1 cell lysate. This image was taken for the unconjugated form of this product. Other forms have not been tested.

Western blot

DHFRL1 antibody LS-C111506 Western blot of THP-1 cell lysate.  This image was taken for the unconjugated form of this product. Other forms have not been tested.
DHFRL1 antibody LS-C111506 Western blot of THP-1 cell lysate. This image was taken for the unconjugated form of this product. Other forms have not been tested.

Western blot

DHFRL1 antibody LS-C111506 Western blot of THP-1 cell lysate.  This image was taken for the unconjugated form of this product. Other forms have not been tested.
DHFRL1 antibody LS-C111506 Western blot of THP-1 cell lysate. This image was taken for the unconjugated form of this product. Other forms have not been tested.


Requested From: 
Date Requested: 6/18/2018

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