Work with LifeSpan to design a custom immunohistochemistry to address your specific biological question. Outsource the entire localization process without having to
worry about finding and characterizing target specific antibodies, sourcing and validating difficult-to-find tissues, and having the ability to interpret the resulting
immunostaining in relation to complex human pathologies.
TCR Screening Services
Test your therapeutic antibodies in immunohistochemistry against a broad panel of normal frozen human tissue types in order to determine potential unintended binding.
Our non-GLP TCR services are designed on the FDA recommendation outlined in their "Points to Consider in the Manufacture and Testing of Monoclonal Antibody Products for Human Use".
Human (tested or 100% immunogen sequence identity)
Specificity and Use
DEPDC4 antibody was raised against synthetic peptide C-EPPVKTQNLQLN from an internal region of human DEPDC4 (NP_689530.1). Percent identity by BLAST analysis: Human, Gorilla, Gibbon (100%); Horse (83%).
Western Blot: Preliminary experiments gave an approx 75kD band in Human Brain (Amygdala) lysates and in lysates of cell lines A431 and KELLY after 1 ug/ml antibody staining. Please note that currently we cannot find an explanation in the literature for the band we observe given the calculated size of 33.7kD according to NP_689530.1. The 75kD band was successfully blocked by incubation with the immunizing peptide. Have any further splice variants/modified forms been reported?