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Work with LifeSpan to design a custom immunohistochemistry to address your specific biological question. Outsource the entire localization process without having to worry about finding and characterizing target specific antibodies, sourcing and validating difficult-to-find tissues, and having the ability to interpret the resulting immunostaining in relation to complex human pathologies.

Test your therapeutic antibodies in immunohistochemistry against a broad panel of normal frozen human tissue types in order to determine potential unintended binding. Our non-GLP TCR services are designed on the FDA recommendation outlined in their "Points to Consider in the Manufacture and Testing of Monoclonal Antibody Products for Human Use".

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DENND4C Antibody (aa31‑45) LS‑C139723 Best Seller
DENND4C antibody LS-C139723 is an unconjugated goat polyclonal antibody to DENND4C from human and mouse. Validated for Peptide-ELISA and WB.
Catalog
Size
Price
LS-C139723-100
100 µg (0.5 mg/ml)
Unavailable

Popular DENND4C Products

Western blot of Human Jurkat. DENND4C antibody dilution 1.0 ug/ml.  This image was taken for the unconjugated form of this product. Other forms have not been tested.
Species: Human, Bovine, Dog, Horse
Applications: Western blot
Western blot of Human Jurkat. DENND4C antibody dilution 1.0 ug/ml.  This image was taken for the unconjugated form of this product. Other forms have not been tested.
Species: Human, Chimpanzee, Orangutan, Gibbon, Bovine, Dog, Horse
Applications: Western blot
Western blot of Human Jurkat. DENND4C antibody dilution 1.0 ug/ml.  This image was taken for the unconjugated form of this product. Other forms have not been tested.
Species: Human, Chimpanzee, Orangutan, Gibbon, Bovine, Dog, Horse
Applications: Western blot
Western blot of Human Jurkat. DENND4C antibody dilution 1.0 ug/ml.  This image was taken for the unconjugated form of this product. Other forms have not been tested.
Species: Human, Chimpanzee, Orangutan, Gibbon, Bovine, Dog, Horse
Applications: Western blot

Product Description

DENND4C antibody LS-C139723 is an unconjugated goat polyclonal antibody to DENND4C from human and mouse. Validated for Peptide-ELISA and WB.
About DENND4C
Guanine nucleotide exchange factor (GEF) activating RAB10. Promotes the exchange of GDP to GTP, converting inactive GDP-bound RAB10 into its active GTP-bound form. Thereby, stimulates SLC2A4/GLUT4 glucose transporter-enriched vesicles delivery to the plasma membrane in response to insulin. Q5VZ89 NM_017925 NP_060395.5

DENND4C Antibody, BA513M16.3 Antibody, C9orf55B Antibody, C9orf55 Antibody, DENN/MADD domain containing 4C Antibody


Specifications

Target
Human DENND4C
Host
Goat
Reactivity
Human, Mouse (tested or 100% immunogen sequence identity)
Clonality
Polyclonal
Conjugations
Unconjugated
Purification
Immunoaffinity purified
Modifications
Unmodified
Applications
  • Western blot (0.5 - 2 µg/ml)
  • Peptide Enzyme-Linked Immunosorbent Assay (1:8000)
Immunogen
DENND4C antibody was raised against synthetic peptide C-RFKPQSPYRAYKDRS from an internal region of human DENND4C (NP_908976.1; NP_001074483.1). Percent identity by BLAST analysis: Mouse (100%); Hamster (87%).
Blocking Peptide
LS-E27577 - Lyophilized - 100 µg - $145.00
Immunizing peptide used to generate LS-C139723. Useful for pre-absorption and neutralization of the antibody's antigen binding site.
Epitope
aa31-45
Specificity
Human DENND4C. This antibody is expected to recognize both reported isoforms (NP_908976.1; NP_001074483.1).
Usage
Peptide ELISA: antibody detection limit dilution 1:8000. Western blot: Approx 210kD band observed in Mouse Spleen lysates (calculated MW of 211kD according to NP_908976.1). Recommended concentration: 0.5-2 ug/ml.
Presentation
Tris-buffered saline, pH 7.3, 0.5% BSA, 0.02% sodium azide
Storage
Store at -20°C. Avoid freeze-thaw cycles.
Restrictions
For research use only.
Guarantee
This antibody carries the LSBio 100% Guarantee.

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Images


Western blot

DENND4C antibody (0.5 ug/ml) staining of Mouse Spleen lysate (35 ug protein/ml in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
DENND4C antibody (0.5 ug/ml) staining of Mouse Spleen lysate (35 ug protein/ml in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

Western blot

DENND4C antibody (0.5 ug/ml) staining of Mouse Spleen lysate (35 ug protein/ml in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
DENND4C antibody (0.5 ug/ml) staining of Mouse Spleen lysate (35 ug protein/ml in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

Western blot

DENND4C antibody (0.5 ug/ml) staining of Mouse Spleen lysate (35 ug protein/ml in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
DENND4C antibody (0.5 ug/ml) staining of Mouse Spleen lysate (35 ug protein/ml in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

Western blot

DENND4C antibody (0.5 ug/ml) staining of Mouse Spleen lysate (35 ug protein/ml in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
DENND4C antibody (0.5 ug/ml) staining of Mouse Spleen lysate (35 ug protein/ml in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.


Requested From: 
Date Requested: 6/21/2018

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