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Work with LifeSpan to design a custom immunohistochemistry to address your specific biological question. Outsource the entire localization process without having to worry about finding and characterizing target specific antibodies, sourcing and validating difficult-to-find tissues, and having the ability to interpret the resulting immunostaining in relation to complex human pathologies.

Test your therapeutic antibodies in immunohistochemistry against a broad panel of normal frozen human tissue types in order to determine potential unintended binding. Our non-GLP TCR services are designed on the FDA recommendation outlined in their "Points to Consider in the Manufacture and Testing of Monoclonal Antibody Products for Human Use".

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DDX23 / PRP28 Antibody (C‑Terminus) LS‑C31429
PRP28 antibody LS-C31429 is an unconjugated rabbit polyclonal antibody to PRP28 (DDX23) from human, mouse, rat and other species. Validated for WB.
Catalog
Size
Price
LS-C31429-100
100 µg
$325

Popular DDX23 / PRP28 Products

DDX23 Antibody IHC of formalin-fixed and paraffin-embedded lung carcinoma followed by peroxidase-conjugated secondary antibody and DAB staining.
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Detection of Human DDX23 by Immunohistochemistry. Sample: FFPE section of human breast carcinoma. Antibody: Affinity purified rabbit anti-DDX23 used at a dilution of 1:200 (1 ug/ml). Detection: DAB.
Species: Human, Mouse, Rat, Bovine, Dog, Guinea pig, Horse, Pig, Rabbit
Applications: IHC, IHC - Paraffin, Immunofluorescence, Western blot, Immunoprecipitation
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Species: Human
Applications: IHC, Western blot, Flow Cytometry, ELISA
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Species: Human
Applications: IHC, Western blot, Flow Cytometry, ELISA

Product Description

PRP28 antibody LS-C31429 is an unconjugated rabbit polyclonal antibody to PRP28 (DDX23) from human, mouse, rat and other species. Validated for WB.

Specifications

Target
Human DDX23 / PRP28
Synonyms
DDX23, DEAD box protein 23, PRP28 homolog, yeast, PRPF28, Prp28, U5-100KD, U5 snRNP 100 kD protein, PRP28 homolog, PRP28p homolog, U5-100K
Host
Rabbit
Reactivity
Human, Monkey, Mouse, Rat, Bovine, Dog, Guinea pig, Hamster, Horse, Pig, Rabbit, Xenopus, Zebrafish (tested or 100% immunogen sequence identity)
Clonality
IgG Polyclonal
Conjugations
Unconjugated. Also available conjugated with FITC, Biotin, HRP.
Purification
Protein A purified
Modifications
Unmodified
Applications
  • Western blot (1.25 µg/ml)
  • (applications tested for the base form of this product only)
Immunogen
DDX23 / PRP28 antibody was raised against synthetic peptide from C-Terminus of human DDX23 (Q9BUQ8, NP_004809). Percent identity by BLAST analysis: Human, Chimpanzee, Gorilla, Orangutan, Monkey, Galago, Marmoset, Mouse, Rat, Hamster, Elephant, Panda, Dog, Bovine, Rabbit, Horse, Pig, Guinea pig, Platypus, Xenopus, Zebrafish (100%); Stickleback (92%).
Blocking Peptide
LS-E13025 - Lyophilized - 100 µg - $145.00
Immunizing peptide used to generate LS-C31429. Useful for pre-absorption and neutralization of the antibody's antigen binding site.
Epitope
C-Terminus
Specificity
Human DDX23
Presentation
Lyophilized from PBS, 0.09% sodium azide, 2% sucrose
Reconstitution
Centrifuge the vial prior to opening. Reconstitute with sterile distilled water to a concentration of 1 mg/ml. Vortex and centrifuge again.
Storage
Long term: -20°C, the use of 50% glycerol is recommended if storing aliquots in -20°C for long term use (up to 1 year); Short term (less than 1 week): 4°C. Avoid freeze-thaw cycles.
Restrictions
For research use only.
About DDX23 / PRP28
DDX23 / PRP28 is a member of the DEAD box protein family. DEAD box proteins, characterized by the conserved motif Asp-Glu-Ala-Asp (DEAD), are putative RNA helicases. They are implicated in a number of cellular processes involving alteration of RNA secondary structure, such as translation initiation, nuclear and mitochondrial splicing, and ribosome and spliceosome assembly. Q9BUQ8 NM_004818 NP_004809.2


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Images

Western blot

HepG2 Cell Lysate.  This image was taken for the unconjugated form of this product. Other forms have not been tested.
HepG2 Cell Lysate. This image was taken for the unconjugated form of this product. Other forms have not been tested.

Western blot

HepG2 Cell Lysate.  This image was taken for the unconjugated form of this product. Other forms have not been tested.
HepG2 Cell Lysate. This image was taken for the unconjugated form of this product. Other forms have not been tested.

Western blot

HepG2 Cell Lysate.  This image was taken for the unconjugated form of this product. Other forms have not been tested.
HepG2 Cell Lysate. This image was taken for the unconjugated form of this product. Other forms have not been tested.

Western blot

HepG2 Cell Lysate.  This image was taken for the unconjugated form of this product. Other forms have not been tested.
HepG2 Cell Lysate. This image was taken for the unconjugated form of this product. Other forms have not been tested.

Requested From: United States
Date Requested: 10/23/2018

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