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DAZ4 Antibody (aa179‑228) LS‑C110499
DAZ4 antibody LS-C110499 is an unconjugated rabbit polyclonal antibody to human DAZ4. Validated for WB.
Catalog
Size
Price
LS-C110499-50
50 µg
Unavailable

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Product Description

DAZ4 antibody LS-C110499 is an unconjugated rabbit polyclonal antibody to human DAZ4. Validated for WB.
About DAZ4
DAZ4 is a member of the DAZ gene family and is a candidate for the human Y-chromosomal azoospermia factor (AZF). Its expression is restricted to premeiotic germ cells, particularly in spermatogonia. It encodes an RNA-binding protein that is important for spermatogenesis. Four copies of this gene are found on chromosome Y within palindromic duplications; one pair of genes is part of the P2 palindrome and the second pair is part of the P1 palindrome. Q86SG3 NM_001005375 NP_001005375.1

DAZ4 Antibody, Deleted in azoospermia 4 Antibody, PDP1680 Antibody, PDP1681 Antibody


Specifications

Target
Human DAZ4
Host
Rabbit
Reactivity
Human (tested or 100% immunogen sequence identity)
Predicted
Monkey, Mouse, Rat, Bovine, Guinea pig, Horse, Pig (at least 90% immunogen sequence identity)
Clonality
Polyclonal
Conjugations
Unconjugated. Also available conjugated with Biotin, FITC, HRP.
Purification
Immunoaffinity purified
Modifications
Unmodified
Applications
  • Western blot (0.2 - 1 µg/ml)
  • (applications tested for the base form of this product only)
Immunogen
DAZ4 antibody was raised against synthetic peptide located between aa179-228 of human DAZ4 (Q86SG3-2, NP_065153). Percent identity by BLAST analysis: Human (100%); Chimpanzee, Gorilla, Monkey, Galago, Marmoset, Tamarin, Mouse, Rat, Elephant, Bovine, Horse, Pig, Guinea pig (92%); Gibbon (85%).
Blocking Peptide
LS-E14650 - Lyophilized - 100 µg - $145.00
Immunizing peptide used to generate LS-C110499. Useful for pre-absorption and neutralization of the antibody's antigen binding site.
Epitope
aa179-228
Specificity
Human DAZ4
Usage
Western Blot: Suggested dilution at 1 ug/ml in 5% skim milk / PBS buffer, and HRP conjugated anti-Rabbit IgG should be diluted in 1: 50,000 - 100,000 as secondary antibody.
Presentation
Lyophilized from PBS with 2% sucrose
Reconstitution
Distilled water
Storage
Long term: -20°C, the use of 50% glycerol is recommended if storing aliquots in -20°C for long term use (up to 1 year); Short term (less than 1 week): 4°C. Avoid freeze-thaw cycles.
Restrictions
For research use only.
Guarantee
This antibody carries the LSBio 100% Guarantee.

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Images


Western blot

DAZ4 antibody LS-C110499 Western blot of HT1080 cell lysate.  This image was taken for the unconjugated form of this product. Other forms have not been tested.
DAZ4 antibody LS-C110499 Western blot of HT1080 cell lysate. This image was taken for the unconjugated form of this product. Other forms have not been tested.

Western blot

DAZ4 antibody LS-C110499 Western blot of HT1080 cell lysate.  This image was taken for the unconjugated form of this product. Other forms have not been tested.
DAZ4 antibody LS-C110499 Western blot of HT1080 cell lysate. This image was taken for the unconjugated form of this product. Other forms have not been tested.

Western blot

DAZ4 antibody LS-C110499 Western blot of HT1080 cell lysate.  This image was taken for the unconjugated form of this product. Other forms have not been tested.
DAZ4 antibody LS-C110499 Western blot of HT1080 cell lysate. This image was taken for the unconjugated form of this product. Other forms have not been tested.

Western blot

DAZ4 antibody LS-C110499 Western blot of HT1080 cell lysate.  This image was taken for the unconjugated form of this product. Other forms have not been tested.
DAZ4 antibody LS-C110499 Western blot of HT1080 cell lysate. This image was taken for the unconjugated form of this product. Other forms have not been tested.


Requested From: 
Date Requested: 6/22/2018

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