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Anti-CYC1 / Cytochrome C-1 Antibody (C-Terminus) LS-C111355

Catalog	Size	Price
LS-C111355-50	50 µg (1 mg/ml)	Unavailable

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Anti-CYC1 / Cytochrome C-1 Antibody (aa259-287, PE) LS-C208740

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Application:	IHC, Western blot, Flow Cytometry, ELISA
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Anti-CYC1 / Cytochrome C-1 Antibody (aa267-297) LS-C320710

Antibody:	Rabbit Polyclonal (IgG) to Human CYC1 / Cytochrome C-1
Reactivity:	Human
Application:	IHC, Western blot, Flow Cytometry, ELISA
Format:	Unconjugated

Antibody:	Rabbit Polyclonal to Human CYC1 / Cytochrome C-1
Reactivity:	Human, Monkey, Mouse, Rat, Bovine, Dog, Guinea pig, Horse, Pig
Application:	Western blot (applications tested for the base form of this product only)
Format:	Unconjugated

Details

Target:	Human CYC1 / Cytochrome C-1
Host:	Rabbit
Reactivity:	Human, Monkey, Mouse, Rat, Bovine, Dog, Guinea pig, Horse, Pig (tested or 100% immunogen sequence identity)
Predicted:	Xenopus, Zebrafish (at least 90% immunogen sequence identity)
Clonality:	Polyclonal
Conjugations:	Unconjugated. Also available conjugated with Biotin, FITC, HRP.
Purification:	Immunoaffinity purified
Modifications:	Unmodified

Applications

Approved:

Western blot (0.2 - 1 µg/ml)
(applications tested for the base form of this product only)

Specificity and Use

Immunogen:

CYC1 / Cytochrome C-1 antibody was raised against synthetic peptide from C-Terminus of human CYC1 (P08574, NP_001907). Percent identity by BLAST analysis: Human, Chimpanzee, Gorilla, Gibbon, Monkey, Marmoset, Mouse, Rat, Elephant, Dog, Bovine, Horse, Pig, Opossum, Guinea pig (100%); Xenopus, Catfish, Salmon, Stickleback, Pufferfish, Zebrafish (92%).

Blocking Peptide:

LS-E18662 - Lyophilized - 100 µg - $145.00
Immunizing peptide used to generate LS-C111355. Useful for pre-absorption and neutralization of the antibody's antigen binding site.

Epitope:

C-Terminus

Specificity:

Human CYC1

Usage:

Western Blot: Suggested dilution at 1 ug/ml in 5% skim milk / PBS buffer, and HRP conjugated anti-Rabbit IgG should be diluted in 1: 50,000 - 100,000 as secondary antibody.

Packaging

Presentation:	Lyophilized from PBS with 2% sucrose
Reconstitution:	Distilled water
Storage:	Long term: -20°C, the use of 50% glycerol is recommended if storing aliquots in -20°C for long term use (up to 1 year); Short term (less than one week): +4°C. Avoid repeat freeze-thaw cycles.
Restrictions:	For research use only.

About CYC1 / Cytochrome C-1

Uniprot: P08574 NCBI: NM_001916 NP_001907.2

Synonyms: CYC1 Antibody, Complex III subunit 4 Antibody, Cytochrome c-1 Antibody, UQCR4 Antibody, Complex III subunit IV Antibody

Description: This is the heme-containing component of the cytochrome b-c1 complex, which accepts electrons from Rieske protein and transfers electrons to cytochrome c in the mitochondrial respiratory chain.

(More About CYC1 / Cytochrome C-1)

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Western blot

CYC1 antibody LS-C111355 Western blot of Jurkat lysate. This image was taken for the unconjugated form of this product. Other forms have not been tested.

Requested From:
Date Requested: 6/27/2017

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