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Anti-CTSS / Cathepsin S Antibody LS-C149895

Catalog Size Price
LS-C149895-25 25 µg Unavailable
LS-C149895-100 100 µg Unavailable

Most Popular CTSS / Cathepsin S Antibodies

Anti-CTSS / Cathepsin S Antibody IHC-plus™ LS-B2550
Goat Polyclonal to Human CTSS / Cathepsin S
Human, Mouse, Rat
IHC - Paraffin, Western blot, Immunoprecipitation
Unconjugated
Immunohistochemistry Image
Anti-CTSS / Cathepsin S Antibody IHC-plus™ LS-B7312
Mouse Monoclonal (IgG2b) to Mouse CTSS / Cathepsin S
Mouse, Human
IHC - Paraffin, ELISA
Unconjugated
Immunohistochemistry Image
Anti-CTSS / Cathepsin S Antibody (aa32-265) LS-C185582
Rabbit Polyclonal (IgG) to Human CTSS / Cathepsin S
Human
IHC - Paraffin, Western blot
Unconjugated
Immunohistochemistry Image
Anti-CTSS / Cathepsin S Antibody (aa298-331) LS-C190578
Rabbit Polyclonal (IgG) to Human CTSS / Cathepsin S
Human, Mouse, Rat
IHC - Paraffin
Unconjugated
Anti-CTSS / Cathepsin S Antibody (aa123-339) LS-C293266
Rabbit Polyclonal (IgG) to Mouse CTSS / Cathepsin S
Mouse
Western blot, ELISA
Unconjugated
Western blot Image

100% Guaranteed
Goat Polyclonal (IgG) to Human CTSS / Cathepsin S
Human
IHC, Western blot, Immunoprecipitation, ELISA, Neutralization
Unconjugated

Details

Human CTSS / Cathepsin S
Goat
Human (tested or 100% immunogen sequence identity)
IgG Polyclonal
Unconjugated
Immunoaffinity purified
Unmodified

Applications

  • IHC
  • Western blot (0.1 - 0.2 µg/ml)
  • Immunoprecipitation (25 µg/ml)
  • ELISA
  • Neutralization

Specificity and Use

NS0-derived rhCathepsin S (aa 17 - 331).
Human Cathepsin S.
Western blot-This antibody can be used at 0.1-0.2 µg/ml with the appropriate secondary reagents to detect human pro and active Cathepsin S. The detection limit for rhCathepsin S is approximately 25 ng/lane under non-reducing and reducing conditions. Immunoprecipitation-This antibody has been used at 25 µg/ml to immunoprecipitate rhCathepsin S from conditioned media of cell culture. Immunohistochemistry-This antibody can be used to detect Cathepsin S in cells and tissues. The recommended working concentration is 5-15 pg/ml. ELISA capture-This product can be used as a capture reagent in a human Cathepsin S sandwich immunoassay in combination with biotinylated human. Cathepsin S detection antibody and recombinant human Cathepsin S as the standard. The suggested coating concentration range is. 0.2-0.8 µg/ml and should be titrated to determine the optimal concentration. In this format, less than 0.05% cross-reactivity with rhCathepsin A, -B, -C, -D, E, L, V, and -X/Z/P was observed. Neutralization-This antibody has been used to neutralize the enzymatic activity of rhCathepsin S. The antibody was preincubated with the activated enzyme at different molar ratios at room temperature for 30 minutes. The enzyme was then assayed with a peptide substrate (7-methoxycoumarin4-yl)acetyl-Arg-Pro-Lys-Pro-Val-Glu-Nva-Trp-Arg-Lys (2, 4-dinitrophenyl)-NH2. 50% of the proteolytic activity was inhibited by the antibody at approximately 1.23 µg/ml (IC50) under conditions in which the enzyme was present at 0.25 µg/ml and the substrate concentration was 10 pM. Considering the molecular masses of the enzyme (30 kD) and the antibody (150 kD), IC50 was achieved at approximately 1:1 molar ratio of the antibody to the enzyme.

Packaging

Lyophilized from 0.2 µm filtered 100 mM NaHCO3, pH 8.3, 500 mM NaCl, 1% sucrose.
Store at -20°C.
For research use only.

About CTSS / Cathepsin S

P25774 NM_004079 NP_004070.3

CTSS Antibody, Cathepsin S Antibody, Cat-s Antibody, CATS Antibody

Thiol protease. Key protease responsible for the removal of the invariant chain from MHC class II molecules. The bond-specificity of this proteinase is in part similar to the specificities of cathepsin L and cathepsin N.

Requested From: 
Date Requested: 10/18/2017

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