Target
Human CTDSPL
Synonyms
CTDSPL | C3orf8 | CTDSP-like | HYA22 | NLI-interacting factor 1 | NIF-like protein | SCP3 | NIF1 | NIFL | Protein YA22 | PSR1 | RBSP3 | Small CTD phosphatase 3 | YA22
Reactivity
Human, Monkey, Bovine
(tested or 100% immunogen sequence identity)
Conjugations
Unconjugated
Purification
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Immunogen
Peptide with sequence QCNVSLKKQRSRS, from the internal region of the protein sequence according to NP_001008393.1NP_005799.2.
Specificity
Human CTDSPL. This antibody is expected to recognise both isoforms (NP_001008393.1; NP_005799.2).
Applications
- Peptide Enzyme-Linked Immunosorbent Assay (1:32000)
Usage
Peptide ELISA: antibody detection limit dilution 1:32000. Western Blot: Preliminary experiments gave an approx 20kD band in human placenta lysates after 1 ug/ml antibody staining. Please note that currently we cannot find an explanation in the literature for the band we observe given the calculated size of 31.1kD according to NP_NP_001008393.1 and of 29.9kD according to NP_005799.2. The 20kD band was successfully blocked by incubation with the immunizing peptide. We would appreciate any feedback from people in the field - have any results been reported with other antibodies / lysates? Have any further splice variants / modified forms been reported?
Presentation
TBS, pH 7.3, 0.02% Sodium Azide, 0.5% BSA
Storage
Aliquot and store at -20°C. Avoid freeze-thaw cycles.
Restrictions
For research use only. Intended for use by laboratory professionals.
Guarantee
This antibody carries the LSBio 100% Guarantee.
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