Target
Human CLDN14 / Claudin 14
Synonyms
CLDN14 | Claudin 14 | DFNB29 | Claudin-14
Reactivity
Human
(tested or 100% immunogen sequence identity)
Conjugations
Unconjugated
Purification
Immunoaffinity purified
Immunogen
Synthetic peptide derived from the mid region of the human Claudin-14 protein.
Specificity
Reacts with the ~22kD human Claudin-14.
Applications
- ICC
- Immunofluorescence (3 - 9 µg/ml)
- Western blot (1 - 3 µg/ml)
Usage
Suitable for use in Immunocytochemistry, Immunofluorescence and Western Blot. Immunofluorescence: 3-9 ug/ml; Immunofluorescence assays were performed using human Claudin 14 transfected-MDCK cells that were fixed with 1% paraformaldehyde, permeabilized with 0.2% Triton X-100 and quenched with 50 mM ammonium chloride. Western Blot: 1-3 ug/ml; Claudin-14 oligomers are observed at above 40kD in Western Blot using HepG2 cell lysates. Positive control: WB: Human HepG2 hepatocellular carcinoma cell lysate.
Presentation
PBS, pH 7.4, 0.1% Sodium Azide, 40% Glycerol
Storage
Store at 4°C or -20°C. Avoid freeze-thaw cycles.
Restrictions
For research use only. Intended for use by laboratory professionals.
Guarantee
This antibody carries the LSBio 100% Guarantee.
LSBio Guarantee
About CLDN14 / Claudin 14