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Anti-ClC-5 / CLCN5 Antibody (C-Terminus) LS-C6131

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Wt. Vol. Conc. Price
- 50 µl - $375
Inquire for larger quantities

LSBio (Direct) LSBio (Direct)
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Rabbit Polyclonal (IgG) to Rat ClC-5 / CLCN5
Rat, Human
Western blot, ELISA
Unconjugated

Details

Rat ClC-5 / CLCN5
Rabbit
Rat, Human (tested or 100% immunogen sequence identity)
IgG Polyclonal
Antiserum

Applications

Western blot (1:1000 - 1:5000), ELISA (1:10000 - 1:50000)

Specificity and Use

ClC-5 / CLCN5 antibody was raised against a 13 AA Peptide sequence near the C-terminus of rat CLC-5 (1). The peptide was coupled to KLH.
C-Terminus
The 13 AA CLC51-P control peptide is 100% conserved in rat and human CLC-5. No significant sequence homology is detected with other CLCs or other proteins. Actual cross-reactivity of antibodies in various species has not been studied. There is a very high degree of protein homology among CLC-3, CLC-4 and CLC-5. However, CLC51-P peptide has very little sequence homology with CLC-3 and CLC-4. Anti-rat CLC41 antibodies were checked against the corresponding CLC-3 and CLC-4. These peptides are also available to confirm specificity of antibodies.
Suitable for use in ELISA, Western Blot. Western Blot: 1:1000-1:5000 for neat serum and 1-10 ug/ml for affinity pure antibody using ECL. Antibodies made to various CLC-5 peptides have been shown to detect a protein of approx. 80kD in kidney. ELISA:1:10000-1:50000 for neat serum and 0.5-1 ug/ml for affinity purified. Control peptide can be used to coat ELISA plates at 1 ug/ml.

Packaging

PBS, 0.05% sodium azide.
Long term: -20°C; Short term: +4°C. Avoid repeat freeze-thaw cycles.
For research use only.

About ClC-5 / CLCN5

P51795 NM_000084 NP_000075.1

CLCN5 Antibody, DENTS Antibody, CLC5 Antibody, HCIC-K2 Antibody, Nephrolithiasis 1 (X-linked) Antibody, Nephrolithiasis 2, X-linked Antibody, NPHL1 Antibody, NPHL2 Antibody, XLRH Antibody, XRN Antibody, Chloride channel 5 Antibody, Chloride channel protein 5 Antibody, Chloride transporter ClC-5 Antibody, ClC-5 Antibody, CLCK2 Antibody, HClC-K2 Antibody

Proton-coupled chloride transporter. Functions as antiport system and exchanges chloride ions against protons. Important for normal acidification of the endosome lumen. May play an important role in renal tubular function.

Requested From: United States
Date Requested: 9/30/2016

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