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Work with LifeSpan to design a custom immunohistochemistry to address your specific biological question. Outsource the entire localization process without having to worry about finding and characterizing target specific antibodies, sourcing and validating difficult-to-find tissues, and having the ability to interpret the resulting immunostaining in relation to complex human pathologies.

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CKLF Antibody (N‑Terminus) LS‑C102274 Best Seller
CKLF antibody LS-C102274 is an unconjugated rabbit polyclonal antibody to human CKLF. Validated for WB.
Catalog
Size
Price
LS-C102274-50
50 µg
Unavailable

Popular CKLF Products

Immunofluorescence of MCF7 cells, using CKLF1 Antibody. The sample on the right was incubated with synthetic peptide.
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Western blot analysis of CKLF1 expression in Jurkat (A); K562 (B); NIH3T3 (C) whole cell lysates.
Species: Human, Mouse
Applications: Western blot
CKLF antibody LS-C102274 Western blot of Jurkat lysate.  This image was taken for the unconjugated form of this product. Other forms have not been tested.
Species: Human
Applications: Western blot

Product Description

CKLF antibody LS-C102274 is an unconjugated rabbit polyclonal antibody to human CKLF. Validated for WB.
About CKLF
CKLF is a cytokine. Cytokines are small proteins that have an essential role in the immune and inflammatory responses. This gene is one of several chemokine-like factor genes located in a cluster on chromosome 16. The protein encoded by this gene is a potent chemoattractant for neutrophils, monocytes and lymphocytes. It also can stimulate the proliferation of skeletal muscle cells. This protein may play important roles in inflammation and in the regeneration of skeletal muscle. Q9UBR5 NM_016326 NP_057410.1

CKLF Antibody, C32 Antibody, Chemokine-like factor 4 Antibody, CKLF4 Antibody, CKLF1 Antibody, CKLF3 Antibody, Chemokine-like factor 1 Antibody, Chemokine-like factor 2 Antibody, Chemokine-like factor 3 Antibody, Chemokine-like factor Antibody, CKLF2 Antibody, HSPC224 Antibody, Transmembrane proteolipid Antibody, UCK-1 Antibody


Specifications

Target
Human CKLF
Host
Rabbit
Reactivity
Human (tested or 100% immunogen sequence identity)
Predicted
Monkey (at least 90% immunogen sequence identity)
Clonality
IgG Polyclonal
Conjugations
Unconjugated. Also available conjugated with Biotin, FITC, HRP.
Purification
Immunoaffinity purified
Modifications
Unmodified
Applications
  • Western blot (0.2 - 1 µg/ml)
  • (applications tested for the base form of this product only)
Immunogen
CKLF antibody was raised against synthetic peptide from N-Terminus of human CKLF (Q9UBR5, NP_001035228). Percent identity by BLAST analysis: Human, Gorilla (100%); Gibbon, Monkey (92%).
Blocking Peptide
LS-E17353 - Lyophilized - 100 µg - $145.00
Immunizing peptide used to generate LS-C102274. Useful for pre-absorption and neutralization of the antibody's antigen binding site.
Epitope
N-Terminus
Specificity
Human CKLF
Usage
Western Blot: Suggested dilution at 1µg/ml in 5% skim milk / PBS buffer, and HRP conjugated anti-Rabbit IgG should be diluted in 1: 50,000 - 100,000 as second antibody. ELISA titer in peptide based assay: 1:312500.
Presentation
Lyophilized from PBS with 2% sucrose
Reconstitution
Distilled water
Storage
Long term: -20°C, the use of 50% glycerol is recommended if storing aliquots in -20°C for long term use (up to 1 year); Short term (less than 1 week): 4°C. Avoid freeze-thaw cycles.
Restrictions
For research use only.
Guarantee
This antibody carries the LSBio 100% Guarantee.

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Images


Western blot

CKLF antibody LS-C102274 Western blot of Jurkat lysate.  This image was taken for the unconjugated form of this product. Other forms have not been tested.
CKLF antibody LS-C102274 Western blot of Jurkat lysate. This image was taken for the unconjugated form of this product. Other forms have not been tested.

Western blot

CKLF antibody LS-C102274 Western blot of Jurkat lysate.  This image was taken for the unconjugated form of this product. Other forms have not been tested.
CKLF antibody LS-C102274 Western blot of Jurkat lysate. This image was taken for the unconjugated form of this product. Other forms have not been tested.

Western blot

CKLF antibody LS-C102274 Western blot of Jurkat lysate.  This image was taken for the unconjugated form of this product. Other forms have not been tested.
CKLF antibody LS-C102274 Western blot of Jurkat lysate. This image was taken for the unconjugated form of this product. Other forms have not been tested.

Western blot

CKLF antibody LS-C102274 Western blot of Jurkat lysate.  This image was taken for the unconjugated form of this product. Other forms have not been tested.
CKLF antibody LS-C102274 Western blot of Jurkat lysate. This image was taken for the unconjugated form of this product. Other forms have not been tested.


Requested From: 
Date Requested: 6/24/2018

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