(tested or 100% immunogen sequence identity)
Delipidated and defibrinated
- ICC (1:2500)
- Western blot (1:500)
CHRM3 / M3 antibody was raised against a peptide analogue of the carboxyl terminal of the Muscarinic M3 receptor covalently attached to a carrier protein.
M3 Muscarinic Receptor (580-589) 100% M3 Muscarinic Receptor 70% M1 Muscarinic Receptor (451-460) 0% M1 Muscarinic Receptor 0% M2 Muscarinic Receptor (457-466) 0% M2 Muscarinic Receptor 0% M4 Muscarinic Receptor (469-478) 0% M4 Muscarinic Receptor 0% M5 Muscarinic Receptor (419-531) 0% M5 Muscarinic Receptor 0%.
Suitable for Immunocytochemical and Western Blot detection of the receptor. Immunocytochemistry: 1:2500. This product has been found to stain PLP fixed rat brain sections known to express the muscarinic M3 receptor using ABC techniques. Western Blot: 1:500 (Whole rat brain homogenate).
100 µl deionized water
Short term: -20°C; Long term: -20°C.
For research use only.