Work with LifeSpan to design a custom immunohistochemistry to address your specific biological question. Outsource the entire localization process without having to
worry about finding and characterizing target specific antibodies, sourcing and validating difficult-to-find tissues, and having the ability to interpret the resulting
immunostaining in relation to complex human pathologies.
TCR Screening Services
Test your therapeutic antibodies in immunohistochemistry against a broad panel of normal frozen human tissue types in order to determine potential unintended binding.
Our non-GLP TCR services are designed on the FDA recommendation outlined in their "Points to Consider in the Manufacture and Testing of Monoclonal Antibody Products for Human Use".
Mouse Monoclonal [clone MK-302] (IgG1) to Human Chondroitin 6 Sulfate
Human, Mouse, Rat, Rabbit, Sheep
Human Chondroitin 6 Sulfate
Human, Mouse, Rat, Rabbit, Sheep (tested or 100% immunogen sequence identity)
IgG1 Monoclonal [MK-302]
ELISA (1:100 - 1:5000)
Radioimmunoassay (1:100 - 1:200)
Specificity and Use
Chondroitinase ABC-digested adult human aggrecan.
Recognizes human Chondroitin 6 Sulfate. Reacts with a wide range of cartilage proteoglycans after either chondroitinase (ABC or AC) or testicular hyaluronidase digestion. Antibody binding to epitope is successfully inhibited by disaccharides of chondroitin-6-sulfate. Recognizes the chondroitin-6-sulfate stubs (preferentially in clusters) of high density cartilage proteoglycans of different species. Does not react with native proteoglycans, dermatan sulfate, keratan sulfate or chondroitin-4-sulfate. Species cross-reactivity: chondroitin-6-sulfate stubs of all proteoglycans of all species.
Suitable for use in ELISA and RIA. ELISA: 1:100-1:5000. RIA: 1:100-1:200. Chondroitinase ABC digestion prior to antibody reaction is required for antibody reactivity. A dramatic proteolytic digestion of the core protein (e. g. with papain or pronase) significantly reduces the antibody reactivity.