Ammonium sulfate precipitation
Chloramphenicol antibody was raised against aromatic amino derivative of chloramphenicol (prepared by reduction of the nitro group by high-pressure hydrogenation) coupled to KLH, using the diazotization procedure.
Suitable for use in ELISA and Fluoroimmunoassay (FIA). Indirect ELISA: 1:2000; Coat with 400 ng of chloramphenicol-BSA per well. FIA (Polarization Fluoroimmunoassay): 1:750; Tracer is aromatic amino derivative of chloramphenicol labeled by reaction with fluorescein isothiocyanate (10nmol/L).
PBS, pH 7.2, 0.1% sodium azide, 40% glycerol.
For research use only.
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